Folia Medica Indonesiana
ISSN 0303-7932
Vol. 42 / No. 4 / Published : 2006-10
Order : 1, and page :205 - 208
Related with : Scholar Yahoo! Bing
Original Article :
Dna isolation from sweat stain in clothes as forensic identification material
Author :
- Ahmad Yudianto*1
- Soekry Erfan Kusuma*2
- Human Genetic Study Group Airlangga University Tropical Disease Center (TDC)
- Human Genetic Study Group Airlangga University Tropical Disease Center (TDC)
Abstract :
DNA profiling is an instrument to assist the investigation of either criminal or civil cases. It is also highly useful in forensic identification. Until recently, personal identification using sweat stain in clothes with DNA analysis method (DNA profiling) has not been commonly used in Indonesia. A study has been conducted on the sweat stain in clothes using DNA analysis for forensic identification material. Loci in this study were CSF1PO, THO1, TPOX, vWA and D17S5. From 10 samples used, only 6 met the typing requirement (level >20ng/ml and purity 1- 2). As a comparison, we used blood from volunteers. Except the locus D17S5 which was not examined due to the small amount of DNA remains produced from sweat stain, in electrophoresis visualization that presenting as bands, the four loci in sweat and blood stain were identical/consistent. In conclusion, sweat stain in clothes can be used as alternative material for forensic identification using molecular forensic examination.
Keyword :
sweat stain in clothes, STR, forensic identification,
References :
RL Affoed,(1994) DNA analysis in Forensic disease and animal identification no info : Curr Opin Biotech
CT Caskey,(1994) DNA analysis in Forensic disease and animal identification no info : Curr Opin Biotech
B Albert,(1994) Molecular Biology of the Cell New York : Garland Publishing
DS Atmaja,(2005) Peranan sidik jari DNA dalam bidang forensik Yogyakarta : PS Bioteknologi UGM
Becker,(2003) The World of the Cell San Francisco, Boston, New York : Benjamin Cummings
TA Brown,(1991) Pengantar Kloning Gena no info : Yayasan Essentia Medica
E Budianto,(2001) Biostatistika untuk Kedokteran dan Kesehatan Masyarakat no info : Penerbit Buku Kedokteran EGC
JM Butler,(2001) STR analysis for human testing, STR typing no info : Current Protocols in Human Genetic Unit
JM Butler,(2003) Forensic DNA Typing San Diego-Florida : Academic Press
MG Cooper,(2004) The Cell, a Molecular Approach Washington DC : ASM Press
Hausman,(2004) The Cell, a
Archive Article
Cover Media | Content |
---|---|
Volume : 42 / No. : 4 / Pub. : 2006-10 |
|