UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 44 / No. 3 / Published : 2008-07

Order : 3, and page :145 - 149

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Original Article :

Effect of cigarette smoke exposure on the expression of mutant p53 in transitional cells of the bladder an experimental study on rattus norvegicus

Author :

  1. Kristanto RDA*1
  2. Soetojo*2
  3. Soebadi DM*3
  1. Department of Urology, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital., Surabaya, Indonesia
  2. Department of Urology, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital., Surabaya, Indonesia
  3. Department of Urology, Airlangga University School of Medicine, Dr Soetomo Teaching Hospital., Surabaya, Indonesia

Abstract :

We studied the effect of exposure to cigarette smoke on the expression of mutant p53 transitional epithelial cells of the bladder to prove the relation of smoking and bladder cancer. In this experimental study in rat’s (Rattus norvegicus) we used post test control group design. Forty-four rats were allocated randomly into 4 groups; the first 2 groups were exposed to cigarette smoke for 30 days and 60 days respectively, while the other 2 were control groups. All animals were euthanatized and cystectomy was done at the end of the experiment. Bladder slices were examined using HE stain to confirm the histopathologic structure of the bladder, then immunohistochemistry (IHC) stain was applied using p53 monoclonal antibody (Pab 1801). A positive result to IHC stain indicates that the expression of mutant p53 supposed to play a role in the pathophysiology of bladder cancer. Normal transitional cells were found in all animals in each with HE stain, while the IHC stain showed negative results in all control groups and in the 30 days group. However, a positive result on mutant p53 was found in the 60 days group. This study shows that 60 days of cigarette smoke exposure can alter p53 expression in transitional epithelial cells of the bladder.

Keyword :

mutant p53, cigarette smoke, transitional epithelium,


References :

Edward, MM,(2002) Urothelial tumor of the urinary tract, in Campbells Urology, 8th edn Philadelphia : WB Saunders Co

Halliwel, B & Gutteridge,(1999) Free radicals in biology and medicine, in JMC, 3hd edn Oxford : Oxford University Press

Wallace, DMA,(2001) Bladder cancer, in Comprehensive Urology Missouri : Mosby





Archive Article

Cover Media Content

Volume : 44 / No. : 3 / Pub. : 2008-07
  1. Effect Of Hsp70 Expression On T-cd4+ Lymphocyte Apoptosis In Hiv/aids Infection
  2. Ifn-γ In Peripheral Blood Mononuclear Cells And Bronchoalveolar Lavage Patients With Pulmonary Tuberculosis Before And After Treatment With Oral Anti-tuberculosis Drugs
  3. Effect Of Cigarette Smoke Exposure On The Expression Of Mutant P53 In Transitional Cells Of The Bladder An Experimental Study On Rattus Norvegicus
  4. Effect Of High Salt (sodium Chloride) Intake On Fracture Risk In Male White Rats (rattus Norvegicus) An Experimental Laboratory Study
  5. Vibrio Cholerae And Its Typing Profile
  6. Change Of Il-1 Concentration And Intracellular Calcium (ca2+ Ic) As An Indicator Of Increased Bitter Taste In Thypoid Fever
  7. Enriching Communication Behavior Change Framework With Religious Element To Increase Compliance In Taking Anti-tuberculous Drugs
  8. Cognitive Function Disorder As A Reflection Of Immunity Status In People Living With Hiv/aids
  9. Lactate Dehydrogenase Level And The Response Of Non Hodgkin Lymphoma Patients To Chemotherapy Cyclophosphamide Doxorubicin, Vincristine, Prednisone (chop) An Observational, Prospective, Cross-sectional Study At The Department Of Internal Medicine, Dr Soetomo Hospital, Surabaya
  10. Fibular Autostrutgraft As The Optional Treatment Of The Femoral Neck Fractures In Surabaya
  11. The Existence Of A Peak In Adolescent’s Height Increments
  12. Review Article And Clinical Experience: Graves’ Disease And Thyroid Storm: Classical And Non-classical (from Formula G/o: P-39.5 – 120 & Bw-score To Formulas Ts-41668 & Cs-7.1.5)
  13. Review Article: The Effects Of Chlamydia Trachomatis Infection On Women’s Reproduction System

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