UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 2 / Published : 2011-04

Order : 13, and page :137 - 142

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Original Article :

Effect of topical hyaluronate and freeze-dried amnion membrane administration on ck 16 protein expression and the number of epithelial layers in superficial wound of male wistar strain rats

Author :

  1. Imam Susilo*1
  1. Department of Anatomic Pathology, Airlangga University Faculty of Medicine, Surabaya

Abstract :

Wound healing is a dynamic process involving mediators, blood cells, parenchymal cells and extracellular matrix. Cytokeratin (CK) 16 in epidermal wound healing, could be to promote reorganization of the cytoplasmic array of keratin filaments, an event that precedes the onset of keratinocyte migration. The essential components of extracellular matrix is hyaluronic acid, which plays a predominant role in tissue morphogenesis, cell migration, differentiation, and adhesion. The aim of this study was to analyze the effects of Low Molecular Weight Hyaluronate on the total of epithelial layer and expression of CK 16 in wound healing. Superficial-thickness excisional wounds were created along the backs of 32 wistar rats. They were divided into 2 groups. One was treated by freeze-dried amnion and 1% Low Molecular Weight Hyaluronate and the other was treated by freeze-dried amnion only as control group. Each of the groups was divided into 2 sub groups. Each of the sub groups composed of 8 wistar rats based on the periode of termination : 3rd and 7th day after wounded. Histological evaluation was done to measure the total of epithelial layer and expression of CK 16. In conclusion, compound of freeze-dried amnion and low molecular weight hyaluronate improved wound healing and reepithelialization on superficial-thickness excisional wounds.

Keyword :

low molecular weight hyaluronate, wound healing, epithelial layer, CK 16,


References :

Chung JH, Park YK, Paek SM. ,(1999) Effect of Na-Hyaluronan on Stromal and Endothelial Healing in Experimental Corneal Alkali Wound - : J Ophthalmic Res

Fraser JRE, Laurent TC, Laurent UBG. ,(1997) Hyaluronan : its nature, distribution, functions & turnover - : Journal of Internal Medicine

Hamann KJ, Dowling TL, Neeley SP, Grant JA, Leff AR. ,(1995) Hyaluronic Acid enhances cell proliferation during eosinopoiesis through the CD44 surface antigen - : Journal of Immunology





Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2011-04
  1. Immunogenity Of 32.2 Kda Hemagglutinin Bm Protein Of Escherichia Coli Pili Isolated From Infertile Males' Semen
  2. Role Of Ehsp 72 On The Increase Of Il-6 And Nk Cells In Participants Of Majelis Dzikir
  3. Effect Of 2-methoxyethanol On Sperm Count, Leucocyte And Epididymal Epithelial Cell Structure In Rats (rattus Norvegicus)
  4. Reversin Increase The Plasticity Of Bone Marrow-derived Mesenchymal Stem Cell For Generation Of Cardiomyocyte In Vitro
  5. Detection Of Cholera Toxin-producing Vibrio Cholerae Aquatic Isolates In Kalimas River-surabaya
  6. Enzymatic Antioxidant Protection In Physical Exercise With Active And Passive Interval
  7. Review Article: Improving The Quality Of Western Blot Result
  8. Green Clover Potentiates Delaying The Increment Of Imbalance Bone Remodeling Process In Postmenopausal Women
  9. Knee Pain Relieved. The Use Of Extra Anatomy Pathway
  10. Nutritional Condition In Hemodialysis Patients. Differences Between Groups In Surabaya, Indonesia And Juntendo University, Japan
  11. Etiology And Risk Factors For Community Acquired Pneumonia In Dr. Zainoel Abidin Hospital, Banda Aceh
  12. Serological Survey On Avian Influenza Subtype H5n1 Viral Antibody In Healthy Blood Donor In Surabaya
  13. Effect Of Topical Hyaluronate And Freeze-dried Amnion Membrane Administration On Ck 16 Protein Expression And The Number Of Epithelial Layers In Superficial Wound Of Male Wistar Strain Rats

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