UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 3 / Published : 2011-07

Order : 3, and page :154 - 159

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Original Article :

Effectiveness of nuclear dna mini primer set at loci fga, csf1po & d21s11 in high-temperature dna degradation with polymerase chain reaction (pcr) method

Author :

  1. Ahmad Yudianto*1
  2. Theresia Lindawati*2
  3. Pandu Hanindito*3
  1. Department of Forensice Medicine & Medicolegal Faculty of Medicine, Airlangga University
  2. Department of Forensice Medicine & Medicolegal Faculty of Medicine, Airlangga University
  3. Department of Forensice Medicine & Medicolegal Faculty of Medicine, Airlangga University

Abstract :

Forensic identification by DNA testing that can be used to determine the origin of the child; paternity cases; family relationships; and identification of victims is not known, is increasingly recognized in supporting law enforcement in this country. Only in its development with the use of DNA examination is not without problems. One issue that is often a serious problem for forensic DNA experts and other DNA experts is that degraded DNA or condition which is known as DNA degraded. One alternative adopted in the current degradation of DNA by DNA forensic expert is through the use of mini-primer set, namely through the STR ??method of size reduction assays, the examination of nuclear DNA loci. This study uses a mini primer CSF1PO, FGA and D21S11 on molar tooth material with exposure treatment 5000C in 20 and 30 minutes and the temperature of 7500C in 20 and 30 minutes. The results obtained significant decline of  DNA levels in teeth (p <0.05) as the effect of high temperature exposure. Visualization of PCR results obtained are still only CSF1PO loci were detected with a mini primer on the exposure temperature of 7500C for 30 minutes (maximum exposure of this study) so that potential through these loci in the examination of the identification through DNA analysis, especially in a degraded state effects of exposure to high temperatures, as well as speed up the process identification, especially in the event of a disaster (mass disaster) as well as other criminal cases.

Keyword :

effectiveness, DNA degradation, mini primer, high temperature,


References :

Atmaja DS,(2005) Peranan sidik jari DNA pada bidang kedokteran forensic Yogyakarta : UGM

Budowle B and Bieber FR,(2005) Forensic aspects of mass disasters: strategic considerations for DNA-based human identification Tokyo : Legal Med

Burger J, Hummel S, Herrmann B, Henke W,(1999) DNA preservation: A microsatellite-DNA study on ancient skeletal remains - : Electrophoresis

Butler JM, Shen Y, McCord BR,(2004) The Development of Reduced Size STR Amplicons as Tools for Analysis of Degraded DNA - : National Institute of Standards and Technology

Gill P and Whitaker J,(2000) An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA - : For. Sci. Int





Archive Article

Cover Media Content

Volume : 47 / No. : 3 / Pub. : 2011-07
  1. Lung Function Is Declined In Smoking “becak” Drivers
  2. The Increase Of Ehsp 72 In Members Of Dzikir Group
  3. Effectiveness Of Nuclear Dna Mini Primer Set At Loci Fga, Csf1po & D21s11 In High-temperature Dna Degradation With Polymerase Chain Reaction (pcr) Method
  4. The Effect Of Passive Smoking On The Incidence Of Primary Dysmenorrhea
  5. The Role Of Glucose And Phosphate In In Vitro Culture Medium To Overcome Cell Block On Mouse Embryo
  6. Prevalence Of Hepatitis B, Hepatitis C And Human Immunodeficiency Virus Infection Among Pediatric Hematology Oncology Patients With Repeated Transfusion
  7. Clinical Features Of Influenza A (h1n1) In Children At Dr Soetomo General Hospital
  8. Case Report: Pain Relieved In Acute Breast Infection Using Extra Anatomy Pathway
  9. Case Report Growth Hormone Treatment In Short Children. A Report Of 4 Cases
  10. Review Article: Flail Chest Management In Ards
  11. Review Article: Adequate Management Of Depression And Neuropathic Pain In Patient With Type 2 Diabetes

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