UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 50 / No. 1 / Published : 2014-01

Order : 1, and page :1 - 5

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Original Article :

Genetic mutation profile of gyra and parc gene in ciprofloxacin-resistance pseudomonas aeruginosa

Author :

  1. Rosda Febriana*1
  2. Didik Hasmono*2
  3. Kuntaman*3
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran

Abstract :

Pseudomonas aeruginosa is a gram-negative bacterium that continues to be a major cause of opportunistic nosocomial infections, causing around 9–10% of infections in hospital. Nosocomial infections caused by Pseudomonas aeruginosa are frequently life threatening and often challenging to treat. A major reason for its prominence as a pathogen is its high intrinsic resistance to antibiotics. Quinolone resistance is increasing with reported frequencies of 12–20%. In most reported there was a missense mutation in the quinolone target (the gyrA subunit of DNA gyrase) at codon 83 (T83I), although other mutations are sometimes observed. Higher levels of resistance may involve additional mutations in gyrB (DNA gyrase B subunit) or parC (topoisomerase IV). Purpose of this study is to determining the genetic mutation of gyrA gene region and parC in Pseudomonas aeruginosa isolates that resistant to ciprofloxacin. Sensitivity testing of bacteria used broth dilution method (BD Phoenix) and Kirby-Bauer methods; DNA extraction used Extract N-AmpTM Blood PCR Kit; QRDR gyrA and parC amplifications used primer form 1st Base OligonucleotideTM with thermal cycler BioRadicycler; agarose gel elektrophoresis; DNA labelling used QiaQuick PCR Column (QIAGEN, Valecia, CA); DNA sequences used Genetic Analyzer ABI Prisma 310, sequencing result is processed used Genetic program version 9.0 and the result were compared with control. Sequencing results predicted changes of an amino acid in the QRDR of DNA gyrase (gyrA) and topoisomerase IV (parC), all isolates possessed mulitple mutation in gyrA and parC. gyrA gene; Glu-53 →Ser, Thr-83 →Ile, Gly-105 →Ala, Glu-106 →Arg, Ala-136 →Gly, parC gene; Asp-35 →Gly, Asp-62 →Asn, Ser-65 →Ala, Ser-87 →Leu, Ser87 →Trp, and additional silent mutation in all isolates.(FMI 2014;50:1-5)

Keyword :

Pseudomonas aeruginosa, ciprofloxacin, resistance, mutation, gyrA,


References :

Akasaka T, Tanaka M, Yamaguchi A, Sato K,(2001) Type II topoisomerase mutations in fluoroquinolone-resistant clinical strains of Pseudomonas aeruginosa isolated in 1998 and 1999: role of target enzyme in mechanism of fluoroquinolone resistance - : Antimicrob Agents Chemother

Kamatchi C, Magesh H, Sekhar U, Vaidyanathan R,(2009) Identification of clonal cluster of Klebsiella pneumoniae isolates from Chennai by extended spectrum beta lactamase genotyping and antibiotic resistance phenotyping analysis - : American Journal of Infectious Diseases





Archive Article

Cover Media Content

Volume : 50 / No. : 1 / Pub. : 2014-01
  1. Genetic Mutation Profile Of Gyra And Parc Gene In Ciprofloxacin-resistance Pseudomonas Aeruginosa
  2. The Depression Profile Of People Living With Hiv/aids (plwha) Receiving Antiretroviral Treatment In Dr. Soetomo Hospital Surabaya
  3. Agreement Between Estimated Apolipoprotein B-100 Concentration And Apolipoprotein B-100 Concentration Measured By Immunoturbidimetric Method
  4. Er, Pr, And Her-2/neu Profile On Young Breast Carcinoma Patients
  5. Correlation Between Heart Type Fatty Acid Binding Protein Serum Level And Soluble St2 In Acute Coronary Syndrome Patients
  6. Correlation Between N-terminal Pro-brain Natriuretic Peptide Level With Dyastolicfunction Echocardiography Parameter In Acute Coronary Syndrome
  7. Correlation Between E/vp And E/e’ In Left Ventricular Filling Pressure Measurement Among Patients With Dilated Cardiomyopathy
  8. Changes In Platelet Count, Mean Platelet Volume, Platelet Distribution Width, And Plateletcrit In Pulmonary Tuberculosis Severity
  9. Microbial Pattern And Antibiotic Susceptibility Test In Semen Gresik Hospital 2012
  10. Diagnostic Values Of Immature Granulocytes, Eosinopenia And I/t Ratio In Detection Of Early Onset Neonatal Sepsis In Neonates With Bacterial Infection Risk
  11. Effect Of Coffee Drinks To The Reaction Time Of 18-22 Years Old Male University Students
  12. Influence Of Weightlifting On The Emergence Of Partial And Full Thickness Rotator Cuff Tear Detected By Ultrasound Imaging
  13. Mean Score Of Cervical Pedicle Angle To Midline And Inferior End Plate In Adult Using Multi Sliced Computed Tomography

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