UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 45 / No. 3 / Published : 2009-07

Order : 3, and page :185 - 192

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Original Article :

Human sperm preservation using freeze-drying an experimental laboratory study

Author :

  1. MP Budyandini Dyah Pramesti*1
  1. Department of Biomedic, Faculty of Medicine, Airlangga University, Jl Prof Dr Moestopo 47, Surabaya

Abstract :

The objective of this study was to evaluate the application of freeze-drying technique on human sperm preservation and to determine the influence of freeze-drying process and 7 days storage under 4° C temperature on human sperm DNA integrity. The Posttest Only Control Group Design was used in this study. Population was sperm of adult male over 20 years old. The objective of this study was to evaluate the application of freeze-drying technique on human sperm preservation and to determine the influence of freeze-drying process and 7 days under 4° C storage temperature on human sperm DNA integrity. Population of adult male sperm was over 20 years old. The sperm should be in normozoospermia category and was obtained with consideration on WHO method. The sample size was 12, obtained with simple random sampling method. Comet assay was used to determine DNA integrity before freeze-drying, immediately after freeze-drying and 7 days storage after freeze-drying. The sample size was 12, obtained with method of simple random sampling. Comet assay was used to determine DNA integrity before freeze-drying, immediately after freeze-drying and 7 days after freeze-drying storage. Manova was used to test the hypothesis. The results were : no significant difference in DNA integrity between fresh group and freeze-dried group (p>0,05); there was very significant difference in DNA integrity between fresh group or freeze-dried group compared with freeze-dried +7 days storage under 4° C temperature (p<0,01).

Keyword :

Comet Assay, DNA integrity, freeze-drying technique ,


References :

Morris ID, Ilott S, Dixon L and Brison DR,(2002) The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development - : Human Reproduction

Pangestu M,(2002) Preservation of Spermatozoa: Method and Application - : Reprotech





Archive Article

Cover Media Content

Volume : 45 / No. : 3 / Pub. : 2009-07
  1. Comparison Of Apoptotic Index And The Amount Of Brain\'s Pyramidal Neuron Between Newborn Mice Receiving And Not Receiving Docosahexanoic Acid During Pregnancy
  2. Nucleotide Sequence And Sequence Comparison Of The Repeat Sequence 529 (r529) Of An Indonesian Toxoplasma Gondii Isolate
  3. Human Sperm Preservation Using Freeze-drying An Experimental Laboratory Study
  4. Correlation Between P53 Protein Expressions In The Incidence Of Metastasis In Well-differentiated Thyroid Cancer
  5. Hepatoprotective Activity Of Barks Extract Of Six Cinnamomum Species On Carbon Tetrachloride-induced In Albino Rats
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  10. Case Report: Nodular Scleritis With Pre-suspected Retinoblastoma
  11. Acupuncture Therapy To Relieve Pain In Dextral Hypochondrial Area. A Case Study On Referred Pain
  12. Review Article And Clinical Experiences: A Novel Oad With Broadspeactrum Therapeutic Value For Pts With T2dm The Roles Of Galvus® For Pts With T2dm, Mody,txdm, Pre-dm, And T1dm

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