Folia Medica Indonesiana
ISSN 0303-7932
Vol. 48 / No. 3 / Published : 2012-07
Order : 4, and page :109 - 114
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Original Article :
In vitro antibacterial activities of commercial probiotic drinks against salmonella typhii
Author :
- Erien Pradyta*1
- Yuani Setiawati*2
- Priyo Budi Purwono*3
- Mahasiswa Fakultas Kedokteran
- Dosen Fakultas Kedokteran
- Dosen Fakultas Kedokteran
Abstract :
Typhoid fever that caused by Salmonella typhii become serious problem in Indonesia. Besides, knowledge and study about antibacteria activity of probiotic against Salmonella typhii has not been known. The objective of the present study was to evaluate and compare antibacterial activity of probiotic drinks, they are Y and V, that have different probiotic strain against Salmonella typhii, a causative agent of typhoid fever. Equal amount of Y drink or V drink (1 mL) and S. typhii were mixed. One mL of aliquot was transferred to a petri dish added with 19 mL of melted nutrient agar, vortexed, and incubated at 37oC for 24 hours. The procedure was repeated 6 times. The result shows that bacteria colonies grew in nutrition agar plate in all replication of Y and V drinks. The conclusion of this experiment is that in vitro antibacteria activity against Salmonella typhii does not exist and there was no difference between Y and V drinks. The result obtained in this study supported the use of probiotic isolate or bacteriosin isolate (antibacterial agent of probiotic) as next subtance for probiotic test in in vitro studies and we also suggest to undertake in vivo experiment. (FMI 2012;48:109-114)
Keyword :
probiotic, Y drink, V drink, Salmonella typhii, antibacterial activity,
References :
Brooks GF, Butel JS, Morse SA,(2005) Jawetz, Melnick & Adelbergh’s: Mikrobiologi Kedokteran, 1st ed Jakarta : Salemba Medika
Chuayana EL Jr, Ponce CV, Rivera MRB, Cabrera EC,(2003) Antimicrobial activity of probiotics from milk products - : Phil J Microbiol Infect Dis
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