UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 46 / No. 3 / Published : 2010-07

Order : 6, and page :192 - 199

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Original Article :

Inhibition of the mitogen activated protein kinase (mapk) in the inflammatory pain like state using sb 203580 and pd 98059 in mice

Author :

  1. Bambang Subakti Zulkarnain*1
  2. Kirwanto*2
  3. Yulistiani*3
  4. Junaidi Khotib*4
  1. Department of Clinical Pharmacy, Faculty of Pharmacy, Airlangga University
  2. Department of Clinical Pharmacy, Faculty of Pharmacy, Airlangga University
  3. Department of Clinical Pharmacy, Faculty of Pharmacy, Airlangga University
  4. Department of Clinical Pharmacy, Faculty of Pharmacy, Airlangga University

Abstract :

The therapeutic management of pain related inflammation is difficult to handle. The inflammatory process is mediated through the mitogen-activated protein kinase (MAPK) signal transduction pathway. Inhibition of this signal with specific inhibitors of p38 and Erk 1/2 MAPK pathway i.e. SB 203580 and PD 98059 has been reported. This study was aimed to assess the effectiveness of those specific inhibitors for inflammatory pain management in Balb-c mice. Inflammatory model was developed by intraplantar injection of Complete Freud’s Adjuvant (CFA). PD 98059 and SB 203580 were dissolved in 30% DMSO to acquire concentration of 10.0 nmol, then was diluted to obtain doses of 0.1; 1.0 and 5.0 nmol. The intrathecally administered of SB 203580 and PD 98059 was injected once a day for 7 consecutive days at dose of 0.1, 1.0 and 5.0 mmol that was started from day 7 to day 13 after CFA injection. The control group received 10 µl 30% DMSO. Hyperalgesia was measured on day 0,1,3,5,7,8,10,12, and 14 following CFA injection. At dose of 5.0 nmol, PD 98059 increased mice’s latency time to heat stimulation compared with placebo (F(3.26)=6.881; p=0.001). Also, SB 203580 at dose of 0.1, 1.0 and 5.0 showed similar results compared with placebo (F(3.25)=4.394; p=0.002, p=0.001 and p=0.039 for doses 0.1, 1.0 and 5.0 nmol respectively).  MAPK inhibitors such as PD 98059 and SB 203580 can decrease hyperalgesia which is shown by increasing response time toward heat stimuli, so that MAPK inhibitors could have a place of therapy in the inflammatory pain like state due to its effectiveness in decreasing hyperalgesia.

Keyword :

Inflammatory pain, mitogen activated protein kinase, PD 98059, SB 203580, chronic pain,


References :

Hudspith MJ, Siddall PJ, Munglani R,(2003) Physiology of Pain Philadelphia : Lippicott-Raven Publisher

Katzung BG,(2001) Basic and Clinical Pharmacology, 8th ed New York : McGraw-Hill Companies Inc

Kuno M,(1995) The Sinapse: Function, Plasticity, and Neurotrophism New York : Oxford University Press





Archive Article

Cover Media Content

Volume : 46 / No. : 3 / Pub. : 2010-07
  1. Socioeconomic And Genetic Factors Influencing The Strength, Weight, Length And Width Measurements Of Children
  2. Glomerular Filtration Rate In Liver Cirrhosis
  3. Antimicrobial Activity Of Mahkota Dewa [phaleria Macrocarpa (scheff). Boerl.] Leaf Extract Against Pseudomonas Aeruginosa By Agar Dilution And Scanning Electron Microscopy
  4. Mapping Pattern Of Core Dna Loci In Forensic Identification Sample Examination Using Polymerase Chain Reaction (pcr) In Loci Csf1po, Tho1 And Tpox
  5. Spatial Analysis Of Dengue Fever Regarding Its Determinant Factors In Bandar Lampung
  6. Inhibition Of The Mitogen Activated Protein Kinase (mapk) In The Inflammatory Pain Like State Using Sb 203580 And Pd 98059 In Mice
  7. Cloning Of Melanoma Antigen-1 (mage-1) Gene From Fine Needle Aspiration Biopsy Of Hepatic Tissue Of Hepatocelluler Carcinoma Patients
  8. Detection Of Urinary Incontinence Affecting Post-menopausal Age Using Ii Q-7 And Udi-6 Questionnaire At Immanuel Teaching Hospital Bandung
  9. Electron Microscopic Scanning Profile Of Balb/c Mice Intestinal Villi After Per Oral Lps (lipopolysaccharide) During Probiotic Induction
  10. Extensive Intensity Exercise Most Effectively Increases Anaerobic Threshold
  11. Pegagan (centella Asiatica) Extract Increases Vaginal Wall Thickness In Menopausal Rats
  12. Review Article: Interval Exercise With 1:1 Work/rest Ratio Decreases The Risk Factors Of Type-2 Diabetes Mellitus And Coronary Heart Disease
  13. Expression Of Topoisomerase Iiα And Cyclin D1 Proteins In Various Degrees Of Breast Ductal Carcinoma

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