Folia Medica Indonesiana
ISSN 0303-7932
Vol. 49 / No. 2 / Published : 2013-04
Order : 4, and page :88 - 90
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Original Article :
Maternally inherited nucleotide variation of mitochondrial dna (mtdna) d-loop region 126 pb (nt: 34-159 hvs ii) in madurese individuals
Author :
- Ahmad Yudianto*1
- Indah Nuraini M*2
- Dosen Fakultas Kedokteran
- Magister Program on Forensic Science Faculty of Medicine, Airlangga University
Abstract :
Human mtDNA has higher polimorfism level than core-DNA, especially in the D-loop region, which is non coding region and themost polymorphic in the mitochondrial genom. The sequence variation analysis of D-loop region can be used determine theindividual or ethnic , also maternal familiar relationship. the aims of the research, to determinant the nucleotide variation ofmaternal inherited using buccal swab. The method of research: analytic observasional. The result of research, 126 bp (HVSII, nt 59-134) fragment of the D-Loop region were successfully amplified and the found of haplotipe: G101A, G107A, T118A, C129T, T160A.(FMI 2013;49:88-90)
Keyword :
Variants, mitochondrial DNA, D-Loop,
References :
Bodmer WF and Cavalli-Sforza LL,(1976) Genetics, Evolution and Man San Francisco : WH Freeman
Gabriel MN, Huffine EF, Ryan JH, Holland MM, Parsons TJ,(2001) Improved mtDNA sequence analysis of forensic remains using a "mini-primer set" amplification strategy - : J Forensic Sci
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