UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 44 / No. 4 / Published : 2008-10

Order : 2, and page :224 - 232

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Original Article :

Molecular analysis of the coding sequence of mage-1 gene from hepatocelular carcinoma patient and testis

Author :

  1. Gondo Mastutik*1
  2. Sunaryo Hardjowijoto *2
  3. JH Lunardi*3
  4. Ni Nyoman Tri Puspaningsih*4
  5. Soetjipto*5
  1. Department of Anatomical Pathology, School of Medicine, Airlangga University
  2. Department of Urology, Airlangga University School of Medicine
  3. Department of Anatomical Pathology, School of Medicine, Airlangga University
  4. Department of Chemistry, Faculty of Science and Techonology, Airlangga University
  5. Department of Biochemistry, School of Medicine, Airlangga University

Abstract :

The gene MAGE-1 is expressed approximately 60-80% in hepatocellular carcinoma. The gene is also expressed in normal testis. However, molecular analysis of coding area of the gene MAGE-1 from liver tissue of hepatocellular carcinoma patients and normal testicular tissue remains unclear. The objective of this study was to conduct molecular analysis on the coding area of the gene MAGE-1 from liver tissue of hepatocellular carcinoma patients and normal testicular tissue. This was a laboratory explorative study. Samples were obtained from the result of FNAB of the liver tissue from hepatocellular carcinoma patients and normal testicular tissue. RNA was extracted from the sample and then subjected to cDNA synthesis using oligo-T primer. cDNA was used as template for nested PCR. Afterwards, the PCR product was sequenced. Molecular analysis of coding area of gene MAGE-1 was conducted using Clone Manager software and using the site European Bioinformatic Institute http://www.ebi.ac.uk/Tools/clustalw2. Homology analysis of coding area sequence of gene MAGE-1 from normal testicular tissue and the sequence from liver tissue of hepatocellular carcinoma patients had a homology of 100%. The coding sequence of gene MAGE-1 from liver tissue of hepatocellular carcinoma patients and normal testicular tissue had a homology of 100% with sequence accession number M77481, NM_004988, and 99% with sequence accession number BC017555 and AY148486. The whole area of gene MAGE-1 coding could be isolated from the result of FNAB from the liver tissue of patients with hepatocellular carcinoma and those with normal testis using nester PCR, whose the final result presented as a band of +931 pb, containing 927 nucleotides that coded 309 amino acid. The coding area sequence of gene MAGE-1 coding in the liver tissue of hepatocellular carcinoma patients is the same to that in normal testicular tissue (100% homologous).

Keyword :

MAGE-1, molecular analysis, hepatocellular carcinoma, normal testicular tissue,


References :

El-Hoseini, ME, Mohammed, MS, Elshemey, WM, Hussein, TD, Desouky, OS, Elsayed, AA,(2005) Enhanced detection of hepatocellular carcinoma - : Cancer Control

Giardia, E, Capon, F, D'Apice, MR, Amati, A, Arturi, F, Filetti, S, Bonifazi, E, Pucci, S, Conte, C,,(2002) Mutational analysis of peroxiredoxin IV: Exclusion of a positional candidate for multinodular goitre - : BMC Medical Genetics

Liu, BB, Ye, SL, He, P, Liu, YK, Tang, ZY,(1999) MAGE-1 and related MAGE gene expression may be associated with hepatocellular carcinoma - : J Cancer Res Clin Oncol





Archive Article

Cover Media Content

Volume : 44 / No. : 4 / Pub. : 2008-10
  1. Genetic Variation Of G6pd And Adaptive Phenomena In Malarial Infection
  2. Molecular Analysis Of The Coding Sequence Of Mage-1 Gene From Hepatocelular Carcinoma Patient And Testis
  3. Potential Value Of C-reactive Protein As Inflammation Marker And Il-1β, Tnf- As Pro-inflammatory Cytokine On Disease Activity Of Rheumatoid Arthritis
  4. Cell Surface Her2/neu Oncoprotein Expression In Breast Ductal Carcinoma
  5. Difference In The Productivity Of B-lymphocyte Isolated From Lymph Nodes And That Isolated From Mice Spleen In The Production Of Toxocara Cati Monoclonal Antibody Using Hybridoma Technology
  6. Exploration In The Use Of Medicinal Plant Acanthus Ilicifolius L In The Treatment Of Endothelial Dysfunction
  7. Detection Of M. Tuberculosis And Drug Sensitivity Test From Sputum Culture Of Pulmonary Tuberculosis Patients In Dr Soetomo General Hospital Between 2003-2004
  8. The Pharmacokenetics Of Levofloxacin In Healthy Rabbit Treated With Antacid
  9. Potency Of Lactobacillus Reuteri On Reduced Inflammation In Septic Encephalopathy
  10. Pharmacokinetic Parameters Determination Of A Single 500-mg Oral Dose Of Levofloxacin In Healthy Subjects By Using Blood Data
  11. Encephalopathy And Neuroendocrine Disorder In Septic Shock
  12. Osteoarthritic Pain Treatment With Electroacupuncture
  13. Review Article And Clinical Experience: Astaxanthin – Oxidative Stress – Diabetes Mellitus From Basics To Clinics And From General To Specific

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