UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 46 / No. 1 / Published : 2010-01

Order : 9, and page :50 - 59

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Original Article :

Molecular characterization of mexb gene fragment in bandung isolate multiresistant pseudomonas aeruginosa

Author :

  1. Fatmawaty Badaruddin*1
  2. Imam Supardi*2
  3. Usman Chatib Warsa*3
  4. Debbie Soefie Retnoningrum*4
  1. Hasanuddin University
  2. Postgraduate Program, Padjadjaran University
  3. University of Indonesia
  4. Biotechnology KPP, Bandung Institute of Technology

Abstract :

Antibiotics have been used extensively to treat infectious diseases, but now the effectiveness of antibiotic use has declined, this is caused by bacteria that have been resistant to antibiotics. One of them is Pseudomonas aeruginosa which was resistant to several antibio-tick by the pumps MexA-MexB efflux-OprM. Allegedly on mexB gene fragment has the nucleotide sequence changes, which cause amino acid changes, resulting in the recognition of substrates is also changing. Changes in place resulted in recognition of this substrate is not known, and antibiotics are pumped out of bacterial cells, so the concentration of inhibition in the cells will not be achieved. Research conducted observations of the four isolates of P. aeruginosa A, B, C and D obtained from four laboratories in Bandung and test the resistance to several antibiotics (tetracycline, ampicillin, amoxicillin-clavulanic, kanamycin, ciprofloxacin, trimethoprim-sulfamethoxazole, erythromycin and chlor-amfenikol). Minimum Inhibitory Concentration (MIC), the four isolates of P. aeruginosa is 20.57 to 39.07 mg/ml for erythromycin, 29.35 to 48.57 mg/ml for kanamycin, 30.35 to 68.75 mg/ml for tetrasilkin, 45.57 to 97.50 mg/ml for ampicillin, 23.69 to 97.50 mg/ml for chloramphenicol, 25.82 to 59.56 mg/ml for amoxicillin-clavulanic, 21.88 to 79.00 mg/ml for trimethoprim-sulfamethoxazole, and 20.58 to 56.97 mg/ml to ciprofloxacin. Increased MIC of each antibiotic showed the four isolates of P. aeruginosa multiresistance to several antibiotics tested. PCR fragments used for detection of suspected mexB substrate recognition site. Percentage nucleotide sequence homology between mexB isolate of multiresistant and mexB of P. aeruginosa which secrete siderofor pioverdin (L11616, NCBI), showed to isolate 96%, to isolate B 100%, to isolate C 97%, and to isolate D 96%. By using the DNA Star program can be a variation of the gene fragment was detected in four isolates of mexB multiresistance P. aeruginosa isolates A, B, and C and D. These variations cause changes in amino acid isolates A GLI → 417Ser, isolate B → 417Gln Glu, Tre → 424Pro, isolate C Tyr → 328Phe, isolate D Asp → 328His, accompanied by changes in secondary structure, resulting in changes in protein function MexB.

Keyword :

Pseudomonas aeruginosa, mexB, multiresistant,


References :

Alonso, A, Campanario, E & Martines, JL,(1999) Emergence of multidrug-resistant mutants is increased under antibiotic selective pressure in Pseudomonas aeruginosa - : Microbiology

Gotoh, N,(2001) Antibiotic resistance caused by membrane impermeability and multidrug efflux systems - : Nippon Rinsho

Guan, L, Michael, E, Hiroshi, Y & Taiji, N,(1999) Membran topology of the xenobiotic-exporting subunit MexB of the MexA,B- OprM extrusion pump in Pseudomonas aeruginosa - : J Biol Chem





Archive Article

Cover Media Content

Volume : 46 / No. : 1 / Pub. : 2010-01
  1. Comparison Between The Results Of Acid Fast Bacilli Obtained From Spontaneous Sputum With Sputum Induction By Saline 0.9 % And By Salbutamol Nebules In The Diagnosis Of Sputum Smear Negative Pulmonary Tuberculosis
  2. Role Of Free Radicals In Children With Malnutrition
  3. Effect Of Probiotic Administration On The Levels Of Fecal Secretory Immunoglobulin A In Premature Infants
  4. The Implementation Of Hidden Markov Model For Exon Prediction On Dna-gene Plasmodium Falciparum
  5. Correlation Between Cox-2 Expression With Histopathological Grading In Astrocytoma
  6. Effect Of Low Lead Exposure On Gestational Age, Birth Weight And Premature Rupture Of The Membrane
  7. Diagnostic Accuracy Of Pre-operative Fine Needle Aspiration Biopsy In Ameloblastoma
  8. Chemical Analysis Of Loranthaceae Daendropthoe Sp. By Thin Layer Chromatography
  9. Molecular Characterization Of Mexb Gene Fragment In Bandung Isolate Multiresistant Pseudomonas Aeruginosa
  10. Gracilis Muscle Transposition For Traumatic Anal Incontinence A Case Report
  11. Review Article: Management Of Polycystic Ovary Syndrome In Adolescent
  12. The Clinical Features Of Transient Elastography (fibroscan) Examination Among Liver Diseases Patients At Gastroenterohepatology Division Of Dr. Soetomo Hospital, Surabaya

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