UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 45 / No. 3 / Published : 2009-07

Order : 2, and page :180 - 184

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Original Article :

Nucleotide sequence and sequence comparison of the repeat sequence 529 (r529) of an indonesian toxoplasma gondii isolate

Author :

  1. Sri Hartati *1
  2. Asmarani Kusumawati *2
  3. Hastari Wuryastuti *3
  4. Sri Widada*4
  1. Department of Internal Medicine, Faculty of Medicine Airlangga University
  2. Department of Reproduction, Faculty of Veterinary Medicine & Study Center of Biotechnology, University Gadjah Mada, Yogyakarta, Indonesia
  3. Department of Internal Medicine, Faculty of Medicine Airlangga University
  4. UMR 5119, “Pathogens and immunity”, CNRS, Université Montpellier II, 34095 Montpellier Cedex 5, France

Abstract :

Toxoplasmosis is one of the most common parasitic diseases in humans and other warm-blooded animals. In many species of animals, severe disease may result in disastrous effects and important economic consequences. In humans, infections are usually asymptomatic but severe disease can occur in immunocompromized individuals and newborns. Owing to the importance of the disease, the availability of efficient and sensitive early diagnosis is of capital importance. Toxoplasma gondii genome contains repeat sequences and due to their multiple copy number, they constitute ideal targets for genome-based detection methods. The repeat sequence 529 (R529) exhibits the highest copy number within the genome, ranging from 200 to 300 copies. To develop efficient R529-hased diagnosis, it is important to establish its exact nucleotide sequence. We have isolated and amplified the R529 gene from an Indonesian isolate and determined its nucleotide sequence. Sequence comparison was done with two other R529 sequences. Variations were observed.

Keyword :

Diagnosis, Indonesian isolate, repeats sequence R529, sequence comparison, Toxoplasma gondii,


References :

Dubey, J.P. and Beattie, C.P.,(1988) Toxoplasmosis of animals and man Boca Raton : CRC Press

Sri Hartati, Asmarani Kusumawati, Hastari Wuryastuti and Sri Widada, J.,(2006) Primary structure of mature AG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains - : I Vet. Sci.





Archive Article

Cover Media Content

Volume : 45 / No. : 3 / Pub. : 2009-07
  1. Comparison Of Apoptotic Index And The Amount Of Brain\'s Pyramidal Neuron Between Newborn Mice Receiving And Not Receiving Docosahexanoic Acid During Pregnancy
  2. Nucleotide Sequence And Sequence Comparison Of The Repeat Sequence 529 (r529) Of An Indonesian Toxoplasma Gondii Isolate
  3. Human Sperm Preservation Using Freeze-drying An Experimental Laboratory Study
  4. Correlation Between P53 Protein Expressions In The Incidence Of Metastasis In Well-differentiated Thyroid Cancer
  5. Hepatoprotective Activity Of Barks Extract Of Six Cinnamomum Species On Carbon Tetrachloride-induced In Albino Rats
  6. The Efficacy Of Combined Cream Bromelain - Liposome As Enzymatic Debriding Agent For Deep Burn Patients
  7. Determinant Of Social Maturity Level Among The Visitors Of Healthy Infants Clinic
  8. Humanity First, Technology Second. Reducing Infant Mortality Rate With Kangaroo Mother Care. Practical Evidence From South Africa
  9. Identification Of The Victims Of Km Senopati Nusantara Using Dna Profiling. A Case Report Of Disaster Victim Identification
  10. Case Report: Nodular Scleritis With Pre-suspected Retinoblastoma
  11. Acupuncture Therapy To Relieve Pain In Dextral Hypochondrial Area. A Case Study On Referred Pain
  12. Review Article And Clinical Experiences: A Novel Oad With Broadspeactrum Therapeutic Value For Pts With T2dm The Roles Of Galvus® For Pts With T2dm, Mody,txdm, Pre-dm, And T1dm

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