UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 40 / No. 3 / Published : 2004-07

Order : 3, and page :71 - 75

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Original Article :

Polymerase chain reaction (pcr) for the diagnosis of cervical tuberculous lymphadenitis

Author :

  1. Sunarto Reksoprawiro*1
  2. Tjahjo Winantyo*2
  3. Indrayana NS*3
  1. Department of Surgery Airlangga University School of Medicine
  2. Department of Surgery Airlangga University School of Medicine
  3. Airlangga University Tropical Disease Center, Surabaya, Indonesia

Abstract :

Tuberculous lymphadenitis is a common form of extrapulmonary tuberculosis with multiple differential diagnoses. The diagnosis of tuberculosis requires the presence of Mycobacterium tuberculosis by acid-fast staining or bacterial growth in culture. However, these are often difficult in cervical tuberculous lymphadenitis. The objective of this prospective study was to investigate the value of the polymerase chain reaction (PCR) technique for detection of Mycobacterium tuberculosis in the aspirate from fine needle biopsy (FNB) of suspected cervical tuberculous lymphadenitis. The primer to amplify Mycobacterium tuberculosis-complex-specific 123-bp DNA was used. Among 22 cases of cervical tuberculous lymphadenitis diagnosed in clinical situation, Mycobacterium tuberculosis DNA was found by PCR in 21 cases (95.40%). This study concludes that PCR is a useful technique for the demonstration of Mycobacterium tuberculosis DNA fragments in patients with clinically suspected cervical tuberculous lymphadenitis, and its clinical application with FNB could reduce the necessity for open biopsy.

Keyword :

polymerase chain reaction, tuberculosis, lymphadenitis,


References :

Baek CH, Kim SI, Ko YH, Chu KC,(2000) Polymerase chain reaction detection of Mycobacterium tuberculosis from fine-needle aspirate for the diagnosis of cervical tuberculous lymphadenitis - : Laryngoscope

Aljafari AS, Khalil EA, Elsiddig KE, El Hag IA, Ibrahim ME, Elsafi ME, Hussein AM, Elkhidir IM, Sula,(2004) Diagnosis of tuberculous lymphadenitis by FNAC, microbiological methods and PCR: a comparative study - : Cytopathol





Archive Article

Cover Media Content

Volume : 40 / No. : 3 / Pub. : 2004-07
  1. Editorial Vol 40 No 3 2004
  2. Opinion: International Ethical Guidelines For Biomedical Research Involving Human Subjects
  3. Polymerase Chain Reaction (pcr) For The Diagnosis Of Cervical Tuberculous Lymphadenitis
  4. Correlation Between The Methods Of Flowcytometry, Manual Light And Fluorescence Microscopes For Cd4 Cell Count In Normal Adults
  5. Iron Deficiency, Low Arterial Oxygen Saturation And High Hematocrit Level As A Major Micro-environmental Risk Factors In The Development Of Brain Abscess In Patients With Tetralogy Of Fallot
  6. The Effect Of Cellulose On Apoptosis Of Colon Epithelial Cells Of Balb/c Mice That Induced By 9,10-dimethyl-1,2-benz(a)anthracene
  7. Preserved Amniotic Membrane Graft After Bare Sclera Technique With Mitomycin C For Pterygium Surgery
  8. Pharmacodynamic Identification Of Antiseizure Effect Of Ligustrosid Glycoside (a Cns Active Substance) Isolated From Fraxinus Griffithii Clarke On Mice
  9. The Modification Of Osce To Replace The Oral Examination In The Department Of Otorhinolaryngology, Airlangga University School Of Medicine
  10. Body Surface Area (bsa) As The Predictor Of Renal Parenchymal Volume In Healthy Adult
  11. Efficacy Of Ethyl Alcohol Glycerin 69% Handrub In Neonatal Ward Dr. Soetomo Hospital. A Randomised Controlled Trial
  12. Review Article And Clinical Experience: Recent Advances In Diabetes Treatment Options (cardioprotective Novel Oha And Insulin Glargine)
  13. Impacted Button Battery In The Nasal Cavity

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