UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 49 / No. 1 / Published : 2013-01

Order : 4, and page :16 - 20

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Original Article :

Polymerase chain reaction of mycobacterium tuberculosis gyrb gene region for rapid screening test of pulmonary tuberculosis

Author :

  1. Ni Made Mertaniasih*1
  2. Nurul Wiqoyah*2
  3. Kuntaman*3
  4. Manik Retno Wahyunitisari*4
  5. Deby Kusumaningrum*5
  1. Dosen Fakultas Kedokteran
  2. Medical Microbiology Department, Faculty of Medicine, Airlangga University, Dr. Soetomo Hospital, Surabaya
  3. Medical Microbiology Department, Faculty of Medicine, Airlangga University, Dr. Soetomo Hospital, Surabaya
  4. Medical Microbiology Department, Faculty of Medicine, Airlangga University, Dr. Soetomo Hospital, Surabaya
  5. Medical Microbiology Department, Faculty of Medicine, Airlangga University, Dr. Soetomo Hospital, Surabaya

Abstract :

gyrB gene of Mycobacterium tuberculosis have conserved and specific DNA region with high copy number. Primer base in conserved and specific DNA region increases the accuracy of nucleic acid amplification method. Determining of tuberculosis diagnosis accurately and rapidly would be useful to appropriately determine the therapy. The objective of this study was to determine sensitivity and specificity of the PCR of gyrB gene region to detect Mycobacterium tuberculosis compared with gold standard method using Lowenstein Jensen (LJ) medium. Thirty sputum samples from pulmonary Tuberculosis suspects were examined using standard culture method in LJ medium and PCR gyrB gene region. Using PCR we positively found gyrB gene region of Mycobacterium tuberculosis in 21 sputum samples, similar to that if we used the gold standard method, with 100% sensitivity and 100% specificity. In conclusion, PCR of Mycobacterium tuberculosis gyrB gene region has a high accuracy, a rapid screening method for TB laboratory diagnosis.(FMI 2013;49:16-20)

Keyword :

PCR method, gyrB gene region of Mycobactrium tuberculosis,


References :

Kasai H, Ezaki T, Harayama S,(2000) Differentiation of phylogenetically related slowly growing mycobacteria by their gyrB sequences - : J Clin Microbiol

Nagaraja V,(2004) Regulation of DNA topology in mycobacteria - : Current Science





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Volume : 49 / No. : 1 / Pub. : 2013-01
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  4. Polymerase Chain Reaction Of Mycobacterium Tuberculosis Gyrb Gene Region For Rapid Screening Test Of Pulmonary Tuberculosis
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