UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 49 / No. 2 / Published : 2013-04

Order : 5, and page :91 - 96

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Original Article :

Rapid culture method using biphasic media on bacteriologic diagnosis to detect mycobacterium tuberculosis for determining pulmonary tuberculosis

Author :

  1. Ni Made Mertaniasih*1
  2. Lindawati Alimsardjono*2
  3. JF Palilingan*3
  4. Soedarsono*4
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran
  4. Dosen Fakultas Kedokteran

Abstract :

Diagnosis to determine pulmonary TB based on detection of Mycobacterium tuberculosis rapidly and accurately is important todetermine the adequate therapy. Standard culture method using Lowenstein Jensen (L-J) media to detect and diagnoseMycobacterium tuberculosis require long time. Culture on solid clear medium may increase the sensitivity in examining the growthof mycobacterial colonies. Supplemented nutrition of media with sera in agar media and sera plus blood in broth phase media willincrease growth rate of colonies. Selectivity of culture media by added malachite green and penicillin. The objective was todetermine the validity of culture method using a biphasic blood-sera agar medium comparing with the standard culture method L-Jfor the detection of Mycobacterium tuberculosis from sputum specimens of 100 suspect pulmonary TB. The nutrition composition ofbiphasic media blood-sera agar which optimum colony growth of mycobacteria are 3.3gram nutrient agar (Oxoid) in 90ml aquadest,25μg malachite green, 3ml glycerol, 10ml sera, and 100U/ml penicillin, and broth phase contain similar nutrition by added blooddiluted; these biphasic culture method has a high laboratory sensitivity with limit detection of 102cfu/ml and high specificity forMycobacterium tuberculosis. Growth rate of mycobacterial colonies in biphasic media 10-14 days; biphasic method has high clinicalvalidity, 100% sensitivity and spesificity 100% to detect Mycobacterium tuberculosis in sputum of suspect pulmonary TB. Inconclusion, rapid culture method using biphasic media blood-sera agar for bacteriologic diagnosis to detect Mycobacteriumtuberculosis in sputum of suspect pulmonary TB patients have high sensitivity and specificity. (FMI 2013;49:91-96)

Keyword :

rapid culture method, biphasic media, blood-sera agar, Mycobacterium tuberculosis, sputum of pulmonary TB,


References :

Brooks GF, Butel JS, Morse SA,(2004) Jawetz, Melnick & Adelberg’s Medical Microbiology Boston-Toronto : McGraw Hill

Cousins D, Francis B, Dawson D,(1996) Multiplex PCR provides a low-cost alternative to DNA probe methods for rapid identification of Mycobacterium avium and Mycobacterium intracellulare - : J Clin Microbiol





Archive Article

Cover Media Content

Volume : 49 / No. : 2 / Pub. : 2013-04
  1. The Effectiveness Of Topical Garlic Extract For Methicillin Resistant Staphylococcus Aureus Treatment In Burns
  2. Mycobacterium Tuberculosis Immunoglobulin M (igm) And Immunoglobulin G (igg) Survey In Hiv-infected Donors
  3. Comparing The Effect Of Red Yeast Rice, Date Palm, And Guava Leaf Extract On Thrombocyte And Megakaryocyte Count In Thrombocytopenic White Rats
  4. Maternally Inherited Nucleotide Variation Of Mitochondrial Dna (mtdna) D-loop Region 126 Pb (nt: 34-159 Hvs Ii) In Madurese Individuals
  5. Rapid Culture Method Using Biphasic Media On Bacteriologic Diagnosis To Detect Mycobacterium Tuberculosis For Determining Pulmonary Tuberculosis
  6. Effect Of Carbide And Cacl2 Concentration On Vitamin C Contents Of Banana Kepok
  7. Direct Cloning Of Melanoma Antigen 1 (mage-1) And The E2 Gene Of Hog Cholera Virus (hcv) From The Blunt End Pcr Product Using Pet 101/d-topo
  8. Quantitation Of Microorganisms In Lower Respiratory Tract Secretions
  9. Bmd-qus Assessment Result Appropriates To Diagnose Osteoporosis
  10. Acid-fast Bacteria M. Leprae Examination In Leprosy Patients Before And After Treatment
  11. Diagnostic Role Of Intra Operative Ovarian Cyst Fluid Cytology In Determining Malignancy
  12. Correlation Between Adolescents' Knowledge On Drugs Hazard And Their Behavior In Drug Abuse In Tulungagung
  13. Basal Insulin As Add-on Therapy To Oral Glucose-lowering Drugs In Insulin Naive Patients With Type 2 Diabetes Mellitus In Primary Public Health Center

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