UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 48 / No. 1 / Published : 2012-01

Order : 2, and page :6 - 11

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Original Article :

Synthesis of beta fertilin protein polyclonal antibody of human sperm membrane as a candidate for immunocontraceptive material

Author :

  1. Ninik Darsini*1
  2. Hamdani Lunardhi*2
  3. Aucky Hinting*3
  4. Reny I’tishom*4
  5. R. Haryanto Aswin*5
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran
  4. Dosen Fakultas Kedokteran
  5. Dosen Fakultas Kedokteran

Abstract :

Increasing role in male contraception is to achieve better gender equality. Therefore, it is necessary to integrate contraceptive methods for developing alternatives, which until now is very limited because the development of male contraceptive is very low. As a contraceptive method, immunocontraceptive used the principles of immune response induction, with the target of inhibiting receptors binding and ligand that was found in egg and sperm. Male contraceptive methods that had been carried out so far has only 2 types, the use of condom and vasectomy. However, condom effectively prevents pregnancy of only 75-80 percent, while vasectomy will be permanent. So, it is necessary to develop a new contraception that has a long-term efficacy, but reversible as the main cause of azoospermia. This study was to obtain fertilin beta protein polyclonal antibodies from male sperm cell membrane as candidate for immunocontraceptive material, especially for recombinant vaccine development. The method used was explorative-laboratories. The procedures were sperm membrane proteins were isolated with electroelution technique. Then, tests were carried out to the proteins beta fertilin based on pH, temperature and incubation period. Protein isolate resulting from the electroelution was used for confirmation of fertilin beta protein techniques in Western Blot, and immunization of protein isolate beta fertilin to female rabbit, the collection of male beta fertilin polyclonal antibodie, and measurements of beta fertilin antibodies titer. The result showed that male beta fertilin protein from cell sperm membrane had a MW as high as 75 kda. The band found in gel electrophoresis SDS-PAGE was beta fertilin molecular band of 75 kda. Immunization of fertilin beta protein isolate from sperm cell membrane of kda 75 to female rabbits also produced fertilin beta polyclonal antibodies.(FMI 2012;48:6-11)

Keyword :

the antibodies polyclonal fertilin beta, immunocontraception, proteins sperm membrane,


References :

Bohring C, Krause E, Habermann B, Krause W,(2001) Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease - : Mol Hum Reprod

Bandivdekar AH, Vernekar VJ, Kamada M, Raghavan VP,(2005) Antifertility effect of passive administration of antibodies to 80 kDa human sperm antigen and its synthetic peptides in male and female rats - : Am J Reprod Immunol





Archive Article

Cover Media Content

Volume : 48 / No. : 1 / Pub. : 2012-01
  1. Effect Of Sodium Nitrite (nano2) To Erithrocyte And Hemoglobin Profile In White Rat (rattus Norvegicus)
  2. Synthesis Of Beta Fertilin Protein Polyclonal Antibody Of Human Sperm Membrane As A Candidate For Immunocontraceptive Material
  3. Long-term Glucocorticoids Effects Of Bone Lining Cells Apoptosis
  4. Zinc Sulfate Increases Lymphocyte Cd4 Count In Hiv/aids Patients At Icuid Dr. Soetomo Hospital Surabaya
  5. Support Group Effect On Cd4 Levels In Plwha Who Recieved Art In Upipi Rsud Dr. Soetomo Surabaya
  6. Intensity Of Swimming Exercise As Chronic Stress Induction Towards Expression Of Glucose Transporter 1 (glut1) In Brain Capillary Endothelial Of Rats (rattus Norvegicus)
  7. The Training Of Wiggler Monitoring Of Primary School Teachers And Students In Surabaya To Improve Eradication Of Dengue Hemorraghic Fever
  8. Implementation Of Safety Work For Nurse In Handling Hiv/aids Patients
  9. Case Report: Posterior Interosseous Nerve Compression Syndrome

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