UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 40 / No. 3 / Published : 2004-07

Order : 6, and page :90 - 98

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Original Article :

The effect of cellulose on apoptosis of colon epithelial cells of balb/c mice that induced by 9,10-dimethyl-1,2-benz(a)anthracene

Author :

  1. Gondo Mastutik *1
  2. Suhartono Taat Putra *2
  3. Soegeng Soekamto Martoprawiro*3
  1. Department of Anatomic Pathology Airlangga University School of Medicine
  2. Department of Anatomic Pathology Airlangga University School of Medicine
  3. Department of Anatomic Pathology Airlangga University School of Medicine

Abstract :

Incidence and death caused by cancer remains high. Epidemiological studies show that dietary fiber, including cellulose, may reduce the incidence of colon cancer. Cellulose is one of dietary fiber that can not be digested by digestive enzyme but can be fermented by colon microflora to produce butyrate, a short chain fatty acid (SCFA). Butyrate inhibits histone deacetylase (HDAC) enzyme and the process of apoptosis. However, the effect of cellulose on apoptosis of colon epithelial cells are not known. The aim of this study was to prove increasing the number apoptotic colon epithelial cells of BALB/C mice that were fed with cellulose and then were exposed to 9,10-dimethyl-1,2-benz(a) anthracene (DMBA).The design of this experiment was posttest only control group design. The male BALB/C mice of 12 weeks old with body weight of 30 - 40 grams were used in this experiment and were given feed containing 0% or 5% cellulose. Two weeks after cellulose administration the mice were exposed to DMBA and sacrificed 5 days later. The colon was removed and processed in the paraffin block, then was stained with TUNNEL (terminal deoxyuridine nucleotide end labeling) assay technique with apoptag detection kit. The data were analyzed by T-test. Results showed that there was difference (p < 0.05) between the number of apoptotic colon epithelial cells in mice fed with cellulose and those that were not fed with cellulose. The difference was marked by increasing the number of apoptotic colon epithelial cells in mice fed with cellulose. The conclusion of this experiment is that cellulose can increase the number of apoptotic colon epithelial cells of BALB/C mice that are exposed by DMBA. The effect of cellulose on colon carcinogenesis presents as the increasing number of apoptotic colon epithelial cells that are exposed by DMBA.

Keyword :

cellulose, DMBA, apoptosis,


References :

Brody T,(1994) Nutritional Biochemistry Sandiego : Academic Press

Cotran RS, Kumar V, Collins T,(1999) Robbins Pathologic Basic of Disease, 9th ed Philadelphia : WB Saunders Company





Archive Article

Cover Media Content

Volume : 40 / No. : 3 / Pub. : 2004-07
  1. Editorial Vol 40 No 3 2004
  2. Opinion: International Ethical Guidelines For Biomedical Research Involving Human Subjects
  3. Polymerase Chain Reaction (pcr) For The Diagnosis Of Cervical Tuberculous Lymphadenitis
  4. Correlation Between The Methods Of Flowcytometry, Manual Light And Fluorescence Microscopes For Cd4 Cell Count In Normal Adults
  5. Iron Deficiency, Low Arterial Oxygen Saturation And High Hematocrit Level As A Major Micro-environmental Risk Factors In The Development Of Brain Abscess In Patients With Tetralogy Of Fallot
  6. The Effect Of Cellulose On Apoptosis Of Colon Epithelial Cells Of Balb/c Mice That Induced By 9,10-dimethyl-1,2-benz(a)anthracene
  7. Preserved Amniotic Membrane Graft After Bare Sclera Technique With Mitomycin C For Pterygium Surgery
  8. Pharmacodynamic Identification Of Antiseizure Effect Of Ligustrosid Glycoside (a Cns Active Substance) Isolated From Fraxinus Griffithii Clarke On Mice
  9. The Modification Of Osce To Replace The Oral Examination In The Department Of Otorhinolaryngology, Airlangga University School Of Medicine
  10. Body Surface Area (bsa) As The Predictor Of Renal Parenchymal Volume In Healthy Adult
  11. Efficacy Of Ethyl Alcohol Glycerin 69% Handrub In Neonatal Ward Dr. Soetomo Hospital. A Randomised Controlled Trial
  12. Review Article And Clinical Experience: Recent Advances In Diabetes Treatment Options (cardioprotective Novel Oha And Insulin Glargine)
  13. Impacted Button Battery In The Nasal Cavity

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