UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 50 / No. 4 / Published : 2014-10

Order : 2, and page :215 - 218

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Original Article :

The profile of genetic mutation of the gyrb gene on clinical isolation of acinetobacter baumannii resistant to ciprofloxacin

Author :

  1. Marich Amilia Rizka*1
  2. Didik Hasmono*2
  3. Kuntaman*3
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Dosen Fakultas Kedokteran

Abstract :

Nosocomial infection is defined as an infection acquired from a hospital or other health facilities by the patient. One of the causingbacteria is Acinetobacter baumannii. Nosocomial infection therapy caused by said bacteria includes: Using the floroquinoloneantibiotics (especially ciprofloxacin). The resistance is caused by a mutation on the gene that codes the DNA gyrase enzyme (gyrAand gyrB gene). This research was performed to examine the gyrB gene mutation on the clinical isolate, Acinetobacter baumanniithat is resistant to ciprofloxacin by using QiaQuick PCR column (QIAGEN, Valecia, CA) dan Genetic Analyzer ABI Prisma 310.Seven clinical isolates show a resistance against ciprofloxacin phenotype. Base on the sequencing result of the gyrB gene, there is anamino acid change on isolate: number 2 (Glu479  Asp), number 4 (Cys423  Ser and Glu479  Asp), number 5 (Cys423  Ser),number 6 (Glu479  Asp), and number 7 (Leu420  Gln; Cys423  Ser; Leu433  His; and Glu479  Asp), with a missensemutation, a mutation associated with amino acid change. The resistance in this research is caused by the gyrB gene, however,examined through the mutation’s percentage, its role on the resistance is not highly dominant. (FMI 2014;50:215-218)

Keyword :

Acinetobacter baummannii, gyrB gene, mutation, ciprofloxacin,


References :

De la Fuente CM, Dauros SP, Bello TH, Domínguez YM,(2007) Mutations in gyrA and gyrB genes among strains of Gram-negative bacilli isolated from Chilean hospitals and their relation with resistance to fluoroquinolones 135, 1103-1110 : Rev Med Chil

Torres A, Ewig S, Lode H, Carlet J, European HAP working group,(2009) Defining, treating and preventing hospital acquired pneumonia: European perspective 35, 9-29 : Intensive Care Med





Archive Article

Cover Media Content

Volume : 50 / No. : 4 / Pub. : 2014-10
  1. Effect Of Touch Therapy On The Growth Of Toddlers (aged 1-3 Years) With Growth Faltering
  2. The Profile Of Genetic Mutation Of The Gyrb Gene On Clinical Isolation Of Acinetobacter Baumannii Resistant To Ciprofloxacin
  3. Relationship Between Platelet Aggregation And Hs-crp In Patients With Pci Receiving Clopidogrel And Aspirin
  4. Correlation Between Preprocedural Hscrp Level And Ck-mb Ratio Pre And Post Percutaneous Coronary Intervention As A Marker Of Preprocedural Myocardial Injury
  5. Evaluation Of Renal Function In Cervix Cancer Patients By Dose Cisplatin 75 Mg/m2 With Nacl-manitol Hydration
  6. Comparison Of Functional Improvement Scale (nihss) Degree Between Acute Intracerebral Hemorrhage Stroke Patients Receiving Standard Treatment And Those Receiving Standard Treatment Plus Celecoxib
  7. Effectiveness Of Kinesio Taping Compared With Short Wave Diathermy On Pain Perception And Functional Status In Knee Osteoarthritis Patients
  8. Cryptosporidium Action In Fecal And Sputum Samples From Patients With Hiv/aids
  9. Percutaneous Transluminal Angioplasty In Patients With Critical Limb Ischemia
  10. Analysis Of Antibiotic Usage In Patients With Bacteremia In The Icu Unit Of Dr. Soetomo Hospital Surabaya
  11. Analysis Of Blood Sample Lysis Rate On Hemoglobin Examination Results Using Rayto Rt. 7600 Auto Hematology Analyzer
  12. Carotid Artery Stenting In A Patient With Transient Ischemic Attack
  13. Risk Factors Of Low Birth Weight (lbw) Incidence. A Case Control Study

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