UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Medical Microbiology

ISSN

Vol. 1 / No. 1 / Published : 2012-12

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Original Article :

Bactericidal effect of cempedak (artocarpus champeden) stem extract on mycobacterium tuberculosis

Author :

  1. Wirawan Indra Priyantono *1
  2. Ni Made Mertaniasih *2
  3. Danti Nur Indiastuti *3
  1. Faculty of Medicine, Airlangga University, Surabaya
  2. Department of Microbiology, Faculty of Medicine, Airlangga University, Surabaya
  3. Department of Pharmacology and Pharmacy, Faculty of Medicine, Airlangga University, Surabaya

Abstract :

This research aimed to study the bactericidal effect of cempedak (Artocarpus champeden) stem bark extract against Mycobacterium tuberculosis H37Rv in vitro. The cempedak stem bark extract was prepared at the Laboratory of Department of Phytochemistry and Pharmacognosy Faculty of Pharmacy, Airlangga University. The extract was dissolved in DMSO and 0.9% NaCl to achieve a concentration of 500 µg / ml.  Firstly we inoculate the bacterial suspension (concentration = 105 cfu / ml and 104 cfu / ml) in MB 7H9 medium.  Antituberculosis properties tests were conducted by administering the extract solution at concentration of 500 µg / ml to the inoculated MB 7H9 medium. In the control, the bacterial suspension was given with DMSO and 0.9% NaCl. After incubation for 1 week at 37o C and 5% CO2, it was subcultured in MB 7H10 plates and incubated again for 3 weeks at 37o C and 5% CO2. The observations of colonies that grew was confirmed by Ziehl Neelsen acid-fast staining. The results of this study showed no Acid Fast Bacili found in both of bacterial suspension given extract solution. In the controls, on the 105 cfu / ml bacterial suspension was found Acid Fast Bacili. It can be concluded that the stem bark extract of cempedak (Artocarpus champeden) effectively kill the bacterium Mycobacterium tuberculosis H37Rv virulent strain.

Keyword :

Mycobacterium tuberculosis, cempedak stem bark extract, killing effect,


References :

Boonphong,(2007) Antitubercular and Antiplasmodial Prenylated Flavones from the Roots of Artocarpus altilis Chiang Mai : J. Sei





Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2012-12
  1. Antimicrobial Activity Of Hibiscus Sabdariffa Calyx Extract Against Shigella Dysenteriae In Vitro
  2. Bactericidal Effect Of Cempedak (artocarpus Champeden) Stem Extract On Mycobacterium Tuberculosis
  3. Antibacterial Activity Of Papaya Leaves (carica Papaya,l) Infusum Against Escherichia Coli
  4. Ziehl Neelsen Stain Sensitivity In Determining The Etiologic Of Granulomatous Inflammation
  5. Comparative Antibacterial Activity Of Noni Fruit Versus Ciprofloxacin Against Salmonalla Typhii In Vitro
  6. Susceptibility Pattern Of Mycobacterium Tuberculosis From Bronchoalveolar Lavage (bal) Specimen

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