UNIVERSITAS AIRLANGGA



Detail Article

Journal of Basic Medical Veterinary

ISSN 2302-6820

Vol. 4 / No. 1 / Published : 2015-06

Order : 11, and page :70 - 74

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Original Article :

A method of the collection of data comparison immunohisto¬chemistry use photoshop cs5© with the convensional scoring method in an antigen quantification of the virus and to the lungs of h5n1 antigen chtlia chicken

Author :

  1. Andreas Berny Yulianto*1
  1. Dosen FKH Univ. Wijaya Kusuma

Abstract :

In this research result of making data compared between immunohistochemistry using Photoshop CS5©  software remele with a conventional manner use a method that has been modified . Immunohistochemistry preparation used here is the lungs chickens infected AI H5N1 virus and lungs chicken that cytokines ChTL1A express. Both types of this preparation taken the photo using a microscope camera. A digital photograph then processed with Photoshop CS5© then taken to the data in quantification in the form of an antigen pesentase. The results of the adoption of this data then compared with data obtained in the calculation of conventionally. The results from a comparison using correlation analysis shows that there is strong correlation very strong on the calculation count tool use of Photoshop CS5©  compared to hand calculating. While data collection by means of the calculation of the pixels with Photoshop CS5©  histogram show the relation strong correlation .The conclusion of this research is a method of the collection of data with the help of Photoshop CS5© software can be used.  

Keyword :

avian influenza, H5N1, imunohistochemistry, cytokine, ChTL1A, Photoshop CS5©,


References :

Lehr H., D.A.Mankoff, D.Corwin, G. Santeusanio and A.M. Gown.,(1997) Application of Photoshop-based Image Analysis to Quantification of Hormone Receptor Expression in Breast Cancer 45: 1559. : Journal of Histochemistry & Cytochemistry,

Lehr H., D.A.Mankoff, D.Corwin, G. Santeusanio and A.M. Gown.,(1997) Application of Photoshop-based Image Analysis to Quantification of Hormone Receptor Expression in Breast Cancer 45: 1559. : Journal of Histochemistry & Cytochemistry,

Damayanti, R., N.L.P.I. Dharmayanti, R. Indriani, A. Wiyono, Darminto. . ,(2004) Deteksi Virus Avian Influ¬enza Subtipe H5N1 pada Organ Ayam yang Terserang Flu Burung Sangat Patogenik di Jawa Timur dan Jawa Barat dengan Teknik Imunohistokimia, 9 (3) : 197-203 : JITV, .





Archive Article

Cover Media Content

Volume : 4 / No. : 1 / Pub. : 2015-06
  1. Anthelmintic Activity Of Bermuda Grass (cynodon Dactylon) Ethanol Extract On Ascaridia Galli By In Vitro
  2. The Effect Combination Of Guava Leaf (psidium Guajava L.) And Sambiloto Leaf (andrographis Paniculata) On Total And Diffcount Leucocyte Of Mice (mus Musculus) Exposed To Heat Stress
  3. Effect Of Meniran (phyllanthus Niruri, Linn.) Infusion On White Pulp Spleen Diameter Of Vibrio Anguillarum Infected Gouramy Fish
  4. Dipping Of Meniran Infusion (phyllantus Niruri) On Gourame (osphronemus Gouramy) Infected With Vibrio Anguillarum Toward The Level Of Hemoglobin, Packed Cell Volume And Number Of Erythrocytes
  5. Effect Of Propolis Toward The Number And Type Of Blood Leucocyte Count Of Male Mice (mus Musculus)
  6. Effect Of Cigarette Smoke On Type Ii Pycnotic Cells At Intralveolar Septa Of Mice Treated By Aromatherapy Extract Of Hibiscus Sabdariffa Calyx
  7. Immunomodulator Effect Of Sambiloto (andrographis Paniculata) To Total And Differential Counting Of Leucocytes In Mice (mus Musculus) Exposed To Heat Stress
  8. Effect Of Guava Leaves (psidium Guajava L.) Extract On Total And Differential Count Of Leucocytes In Mice (mus Musculus) Exposed To Heat Stress
  9. Most Probable Number (mpn) Of Escherichia Coli Isolated From Water Used By The Food Vendors Around Campus Of Veterinary Medicine Faculty Of Airlangga University
  10. Leukocyte Count And Differential Leukocyte Count Of African Catfish (clarias Gariepinus) After Infected By Aeromanas Hydrophila
  11. A Method Of The Collection Of Data Comparison Immunohisto¬chemistry Use Photoshop Cs5© With The Convensional Scoring Method In An Antigen Quantification Of The Virus And To The Lungs Of H5n1 Antigen Chtlia Chicken
  12. Mozart Classical Music Phenomenon : Study Histology Gyrus Of The Brain As Reflection Of Intelligentsia In The Mus Musculus

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