UNIVERSITAS AIRLANGGA



Detail Article

Journal of Basic Medical Veterinary

ISSN 2302-6820

Vol. 6 / No. 1 / Published : 2017-06

Order : 4, and page :22 - 29

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Original Article :

Immunomodulator effect of mangosteen (garcinia mangostana l.) pericarp extract on macrophage through expression of tlr2 in newcastle disease vaccinated mice

Author :

  1. Hendri Budiyansah*1
  2. Rahaju Ernawati*2
  3. Ngakan Made Rai Widjaja*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The research conducted in a purpose to prove the immunomodulator effect of mangosteen pericarp extract (MPE) through expression of TLR2 in ND vaccinated mice. Animal used in the research were 24 female Balb/c mice, randomly separated into four groups (T0, T1, T2, T3) with six animals each group. T0 group, the control group, was administered with PBS. T1 group was administered with 100 mg/kg of body weight of MPE. T2 group was administered with 200mg/kg of body weight of MPE. T3 group was administered with Stimuno®. All groups were were treated for four days and vaccinated by inactive ND vaccine LaSota strain without any booster on the next day. Blood collection has been done two days after vaccination. The immmunocytochesmistry technique used to stain the cell with TLR2 antibody to observe the macrophages that expressed TLR2 under fluorescent microscope 400x magnification. TLR2 expression cell percentage calculated by formulating TLR2 expression and normal cell. The data analyzed with ANOVA one way and Duncan multiple range test. The result indicated that T0 group has the lowest value of TLR2 expression significantly with T1, T2 and T3 group. T1 group were not significantly different with T2 and T3 group. We can conclude that MPE has immunomodulator effect through expression of TLR2 in ND vaccinated mice and 100mg/ kg of body weight of MPE is the most efficient and effective dose.

Keyword :

mangosteen pericarp extract, immunomodulator effect, TLR2, newcastle disease,


References :

Aher, V.D., A. Wahi, A.M. Pawdey and A. Sonawane,(2011) Antioxidants as Immunomodulator: An Expanding Research Avenue. Int. J. Curr. Pharm Res. 3(1): 8-10 : Pharm

Freshney, R.I,(1986) Animal Cell Culture : A Practical Approach 33-69 : IRL Press

Rantam, F.A,(2003) Metode Imunologi. Surabaya 125-128 : Airlangga University Press.





Archive Article

Cover Media Content

Volume : 6 / No. : 1 / Pub. : 2017-06
  1. Effect Of Moringa Oleifera Leaf Extract On The Histopathological Features Liver Cell Of Male Mice (mus Musculus) Exposed By Methylmercury
  2. Effect Of Concentration And Immersion Time In Suspension Of Soursop Seed (annona Muricata Linn) Flour Against Mortality Of Male Rhipicephalus Sanguineus
  3. Effectiveness Of Moringa Oleifera Leaves Ethanolic Extract As A Hepatoprotective Of Mice (mus Musculus) Induced With Methylmercury
  4. Immunomodulator Effect Of Mangosteen (garcinia Mangostana L.) Pericarp Extract On Macrophage Through Expression Of Tlr2 In Newcastle Disease Vaccinated Mice
  5. The Effect Of Eggplant’s (solanum Melongena L) Powder On Serum Level Glutamate Pyruvate Transaminase (sgpt) And Glutamate Oxaloacetate Transaminase (sgot) Of White Rat (rattus Norvegicus) Blood That Given High Fatty Diet
  6. The Anthelmintic Effectiveness Test Of Bitter Melon Fruit (momordica Charantia L.) Ethanol Extract Againts Mecistocirrus Digitatus In Vitro
  7. Helminthiasis Prevalence Of Pigeons (columba Livia) In Surabaya Through Gastrointestinal Tract Surgery
  8. The Effect Of Gadung (dioscorea Hispida Dennst) Tuber Extract Against Culex Fatigans Mosquito Larvae Mortality By In Vitro
  9. The Effect Of Treated Meniran (phyllanthus Niruri Linn) Plant Infusum On Featuring Liver Histopathology Of Aeromonas Hydrophila Infected In Tilapia (oreochromis Niloticus)
  10. Effect Of Polygonum Minus Leaves Extract On Histopathological Features Of Mice (mus Musculus) Liver Induced By Mercuric Chloride
  11. The Influence Of Brucella Abortus Lipopolysaccharide Subunit Vaccine In Adjuvant Montanide Isa 70 Interleukin-2 Production On Sheeps
  12. Critical Percentage’s Pricing Of 0,001n Format Acid Addition To Cream

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