UNIVERSITAS AIRLANGGA



Detail Article

Journal of Basic Medical Veterinary

ISSN 2302-6820

Vol. 3 / No. 2 / Published : 2014-12

Order : 1, and page :63 - 67

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Original Article :

The study of cross reactivity between h5 clade 2.3.2 serum and h5 clade 2.1.3 virus by using serum neutralization test

Author :

  1. Sigit Wibowo*1
  2. C.A. Nidom*2
  3. Rimayanti*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this study was to find the effectiveness of H5 clade 2.3.2 vaccine to H5 clade 2.1.3 and 2.3.2 virus. The study was located at Avian Influenza Research Center (AIRC) University of Airlangga in the BSL-2 Laboratory. Study was conducted from June to July 2014. Serum of H5 clade 2.3.2 that be used had the titre of 27 by Haemaglutination Inhibition (HI) assay. Serum was tested with both viruses by using serum neutralization (SNT) assay in the MDCK cell culture. The data was analyzed by Reed and Muench Formula. Tissue Culture Infectious Dose 50 (TCID50) of H5 clade 2.1.3 was 1.78 x 108 TCID50/ml and H5 clade 2.3.2 was 5.6 x 107 TCID50/ml. The SNT result showed that 1:100 dilution of serum was able to neutralize H5 clade 2.3.2 virus and protect at least 50% of culture cell. The serum was not able to neutralize H5 clade 2.1.3 virus.    

Keyword :

Avian influenza, H5N1 clade 2.3.2 Vaccine, Serum Neutralization Test (SNT), Cross Reactivity,


References :

Kamps, B. S., Hoffman C. Preiser W. . ,(2006) Influenza Report 2006. Paris. : Flying Publisher. www.InfluenzaReport.com

WHO. ,(2014) Cumulative Number of Confirmed Human Cases for avian Influenza A(H5N1) Amerika : Re-ported to WHO, 2003-2014.

Wibawan IWT. ,(2012) Manifestasi Subklinik Avian Influenza pada Unggas: Ancaman Kesehatan dan Penanggulangannya. Bogor. : Orasi Ilmiah Guru Besar Tetap Fakultas Kedokteran Hewan, Institut Pertanian





Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-12
  1. The Study Of Cross Reactivity Between H5 Clade 2.3.2 Serum And H5 Clade 2.1.3 Virus By Using Serum Neutralization Test
  2. The Inhibition Effect Of Pluchea Indica Less. Leaf Extract To Pasteurella Multocida (in-vitro) By Diffusion Disk Method
  3. Chemopreventive Effect Of Gynura Procumbens (lour) Merr, On The Histopathology Of Mammary Gland In The Rats That Was Induced 12-dimethylbenz(α)antrasen
  4. The Effect Of Meniran (phyllantus Niruri, Linn.) In Increasing The Number Of Total Leukocytes And Leukocyte Counts Of Eimeria Tenella Infected Broiler
  5. Antiviral Effectivity Extract Of Pomegranate Rind (punica Granatum L. ) Against Newcastle Disease Virus In Embryonated Chicken Egg
  6. Profile Of Bone Calcium Levels In The Rat (rattus Norvegicus) After The Repositioning Fractures Of The Femur With Tikel Balung (cissus Quadrangularis) Extract Theraphy
  7. Teat Dipping Effectiveness Using Red Betel (piper Crocatum) Leaf Boiling And Iodine On Growth Of Bacteria
  8. Influence Of Infusa Mangosteen Peel (gracinia Mangostana L) For Blood Glucose Levels Of Hyperglycemic Rats
  9. Potential Of Crude Chlorella As Antibacterial Againts Salmonella Pullorum In Vitro
  10. The Potential Of Red Roses Extract (rosa Damascena Mill) As An Antiseptic Stomatitis In Snakes (python Reticulatus) On The Total Number Leukocyte And Defferential Counting Of Leukocytes

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