Jurnal Ilmiah Perikanan & Kelautan
ISSN 2085-5842
Vol. 1 / No. 2 / Published : 2006-08
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Original Article :
Antibacterial effect of noni fruit (morinda citrifolia l.)extracted by ethanol and extracted by water against aeromonas hydrophila in vitro
Author :
- Rahayu Kusdarwati*1
- Sudarno*2
- Hayu Windyaanita*3
- Staf Pengajar Program Studi Budidaya Perairan
- Dosen Staf Pengajar Program Studi Budidaya Perairan
- Mahasiswa Program Studi Budidaya Perairan
Abstract :
Antibacterial activity of various solvent in noni fruit extracts are different. The experiment was aimed to understand and compare the antibacterial effect of noni fruit (Morinda citrifolia L.) extracted by ethanol and extracted by water against Aeromonas hydrophila in vitro. The experiment was used Completely Randomized Design factorial as the experimental design with ten treatment combination concentration (100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, 0.4% and 0.2%) with three times replication. The test used dilution method with determination of Minimal Inhibitory Concentration Test (MIC Test)and Minimal Bactericidal Concentration Test (MBC Test) and also diffusion method. The result of MBC from noni fruit extracted by ethanol on 1,56% concentration and noni fruit extracted by water on 6,25% concentration was not found any Aeromonas hydrophila bacteria growth. The result of diffusion disc method was the best diameter of inhibitory zone of Aeromonas hydrophila growth from ai treatment (concentration solvent of 100%) was 16,17 mm and bi treatment (noni fruit extracted by ethanol) was 13,07 mm.
Keyword :
antibacterial, Aeromonas hydrophila and extract of noni fruit,
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