UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 7 / No. 1 / Published : 2015-04

Order : 7, and page :39 - 45

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Original Article :

Detection of koi herpesvirus (khv) in nile tilapia (oreochromis niloticus) were infected by artificially infection

Author :

  1. Muhammad Sungging Pradana*1
  2. Suwarno*2
  3. Hari Suprapto*3
  1. Mahasiswa Fakultas Perairan dan Kelautan
  2. Dosen Fakultas Perairan dan Kelautan
  3. Dosen Fakultas Perairan dan Kelautan

Abstract :

Koi Herpesvirus (KHV) was formerly known as Cyprinid herpesvirus-3 (CyHV-3) and carp nephritis interstitial and necrosis gill virus (CNGV) is a virus that infects common carp and koi (Cyprinus carpio and C. carpio koi) in farmed and wild population. KHV cause disease at a temperature of 18-25 °C with mortality rate of 80-90 % in fry and adult fish. Currently KHV also detected in tilapia from the results of monitoring in the field. The presence of KHV in tilapia can occur as a result of maintenance in cages adjacent to the infected carp.KHV diagnostic method currently based on case definition and PCR (Polymerase Chain Reaction) detection. The basic concept of PCR is one DNA molecule is used to produce two copies, then four, then eight and so forth through multiplication by polymerase. PCR results sometimes indicated the presence of a faint band caused a low amount of virus, so it is necessary to investigate the presence of KHV DNA in tilapia using different doses of infection.This study aimed to determine the KHV infectivity in nile tilapia were infected by artificially infection and determine dose KHV infection that can infect nile tilapia. The study design used true experimental with with the presentation of descriptive data. Dose of viral infection are 1 ID50, 10 ID50, 100 ID50 and 1000 ID50.The results showed that no clinical symptoms of KHV infected in nile tilapia. The results of electrophoresis of PCR products showed that the mucus of nile tilapia were infected with a 1000 ID50 immersion dose showed thin bands. The same results are also shown in the gill of nile tilapia infected by gill spray at 1000 ID50 dose. Fish were infected by injection, KHV was not detected in mucus, gill, kidney and liver. The results above show nile tilapia cannot be infected by KHV on various treatment.

Keyword :

KHV, nile tilapia, PCR,


References :

M. El-Matbouli ; H. Soliman,(2011) Transmission of Cyprinid herpesvirus-3 (CyHV-3) from goldfish to naȉve common carp by cohabitation 90: 536-539. : Research in Veterinary Science

Lio-Po, G. D., et. al,(2009) Surveillance of Emerging Fish Viral Pathogens in Some Southeast Asian Countries 61(3): 208-214. : The Israeli Journal of Aquaculture-Bamidgeh

S. Siregar,(2011) Statistika Deskriptif untuk Penelitian. Dilengkapi Perhitungan Normal & Aplikasi SPSS versi 17 324 : Raja Grafindo Persada. Jakarta





Archive Article

Cover Media Content

Volume : 7 / No. : 1 / Pub. : 2015-04
  1. Technology Application Of Liquid Smoke In Processing And Preservation Of Fishery Products In The Mandangin Island
  2. Ibm For Traditional Shrimp Farmers In Pangkah Wetan Village, Ujung Pangkah District, Gresik Region, That Were Not Harvesting In Continuoesly
  3. To Increases The Shrimp Harvesting In Traditional Shrimp Farmer In Permisan Village, Jabon District, Sidoarjo Region That Losted Harvesting For A Long Time By Using Best Management Practise (bmp) Methode
  4. Isolation And Identification Of Vibrio Sp. From Traditional Seafood Products Of Eastern Surabaya City Area
  5. Comparative Morphology Of Blood Cockles In Kenjeran And Sedati
  6. Alternative Feed From Vegetable Waste For Black Tilapia (oreochromis Niloticus)
  7. Detection Of Koi Herpesvirus (khv) In Nile Tilapia (oreochromis Niloticus) Were Infected By Artificially Infection
  8. Seaweed Red Pigment(acanthophora Spicifera) As An Alternative In Producing Natural Dyes Of Catfish (clarias Gariepinus) Sausage Produc
  9. The Effectivety Crude And Soluble Protein Of Myxobolus Koi Spore Againts Intestine Different Degrees In Koi (cyprinus Carpio Koi)
  10. Prevalence And The Survival Rate Of Gold Fish (cyprinus Carpio Linn) That Challenced By Whole Protein Spore Myxobolus Koi In Pond
  11. Increased Nutritional Value Pollard Through Yeast Fermentation Tempe As Artificial Feed Ingredients Tilapia (oreochromis Niloticus)
  12. Immune Response Analysis Of Fish Koi (cyprinus Carpio Koi) Vaccinated Myxobolus Koi Spores Whole Protein As A Vaccine Candidate Myxobolusis
  13. Gis Application To Determine Critical Condition And Rehabilitation Model Of Mangrove Ecosystem In Southern Coast Of Pamekasan Region Madura
  14. Effect Of Red Algae (kappaphycus Alvarezii) On The Quality Of Mackerel (rastrelliger Sp.)
  15. Influence Of Addition Crude Fish Oil (cfo) In White Shrimp (litopenaeus Vannamei) Feed To Cholesterol Content And Fat Retention In Meat
  16. The Effect Of Red Algae Extract (kappaphycus Alvarezii) Against The Total Number Of Bacteria And Organoleptic Value Of Mackerel (rastrelliger Sp.)
  17. Identification And Prevalence Of Helminthic Gastrointestinal In Chub Mackerel (scomber Japonicus) In The Fish Landing Base Muara Angke, North Jakarta
  18. Studies Bioaccumulation Of Lead (pb) In Milkfish (chanos Chanos Forskall) At The Fishponds Around Buntung River, Sidoarjo

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