UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 6 / No. 1 / Published : 2014-04

Order : 1, and page :1 - 6

Related with : Scholar   Yahoo!   Bing

Original Article :

Effect of different glycerol concentration in skim milk and egg yolk diluter for storage process of frozen semen on sperm motility and viability of catfish (pangasius pangasius)

Author :

  1. Endang Dewi Masithah*1
  2. Sudarno*2
  3. Bagus Rizki Novianto*3
  1. Dosen Fakultas Perairan dan Kelautan
  2. Dosen Fakultas Perairan dan Kelautan
  3. Mahasiswa Fakultas Perairan dan Kelautan

Abstract :

Needs of catfish seed always increase every years, especially for farming activities. Fulfillment demand of seed in large quntities and continuing are the main obstacle in the production process of catfish. This can be overcome by the process of cryopreservation. This process requires diluents and cryoprotectants to maintain the fertility of spermatozoa. Skim milk and egg yolks diluents has been used on fish because it was applied to carp and showing a good result of sperm motility and fertilization rate. Glycerol as cryoprotectant was reported effective to be used for European Catfish than the other kind cryoprotectant.This study aimed to determine the effect of different concentrations of glycerol in the diluent skim milk and egg yolk on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. This research method is the experiment with completely randomized design (CRD) as the experimental design. The treatment used is different glycerol concentrations, P1 (11%), P2 (13%), P3 (15%), and P4 (17%) and each of the treatment was repeated 5 times. The main parameters measured were motility (%) and viability (%) of spermatozoa. Supporting parameters include odor, color, pH, viscosity, sperm concentration and catfish (Pangasius pangasius). Analysis of the data using Analysis Of Variance (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test with 95% confidance interval.The results showed that the effect of the concentration of glycerol in the diluent skim milk and egg yolk was not significantly (P> 0.05) on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. Further testing needs to be done on the level of fertility of frozen sperm, as well as the growth rate of seeds produced from sperm frozen catfish (Pangasius pangasius) products.

Keyword :

Frozen Semen, Catfish, Cryoprotectant, Glycerol,


References :

C. Saparinto,(2010) Usaha Ikan Konsumsi di Lahan 100 m2 X : Penebar Swadaya. Jakarta.





Archive Article

Cover Media Content

Volume : 6 / No. : 1 / Pub. : 2014-04
  1. Effect Of Different Glycerol Concentration In Skim Milk And Egg Yolk Diluter For Storage Process Of Frozen Semen On Sperm Motility And Viability Of Catfish (pangasius Pangasius)
  2. Substitution Of Soybean Meal With Sword Bean Seed Meal (canavalia Ensiformis) On Growth, Survival Rate And Feed Efficiency Of Red Tilapia
  3. Monitoring Of Fishery With Fishing Gear Troling Line In The Prigi Nusantara Fishing Port, Trenggalek District, East Java Province
  4. Total Of Bacteria In Digestive Organ Of Gurami Fish (osphronemus Gouramy) By Administering A Different Commercial Feed
  5. Albumin Level Test And Snakehead Fish (channa Striata) Growth With Different Commercial Feed Protein Level
  6. Ibm For Seed Shrimp Farmers Family Scale (backyard) In Kalitengah Village, Tanggulangin District, Sidoarjo Region, That Harvesting Lossed To Long Times That Caused By The Diseases
  7. Antifungal Potential Test Of Celery Leaves Juice (apium Graveolens L) Against Aspergilllus Terreus By In Vitro
  8. Metal Detection Of Lead In Tilapia (oreochromis Niloticus) Along The Kalimas River In Surabaya
  9. The Present Effect Of Different Probiotics On Commercial Feed Towards Growth And Feed Efficiency Of Sangkuriang Catfish (clarias Sp.)
  10. The Effect Of Stocking Density On Survival Rate And Grow Rate Of Aquaponic System
  11. Ibm For Traditional Shrimp Farmers In Masaran Village, Banyuates District, Sampang Region, That Capital Losted By Shrimp Dead Cases Continuoesly
  12. Morphological Variation Of Lernaea Holdfast Infest Gouramy In East Java
  13. Digestibility Value Of Crude Fiber And Nitrogen Free Extract (nfe) For Different Commercial Feed In Gouramy (osphronemus Goramy Lac.) Using Surgical Technique
  14. Bioactivity Essential Oil Of Bandotan Leaf (ageratum Conyzoides) On Koi (cyprinus Carpio) Physiological Responses (blood Glucose And Tachiventilation)
  15. Isolation And Identification Of Bacterial In The Digestive Organ Of Gurami Fish (osphronemus Gouramy) With Different Commercial Feed
  16. The Secondary Metabolites Potential Of Infusum Durian’s (durio Zibethinus) Leaves Effect To Survival Rate Of Nila (oreochromis Niloticus) On The Livefish Dry Transport System
  17. Analysis Of Heavy Metal Content Of Lead (pb) And Cadmium (cd) Shells On Green (perna Viridis L.) On Water District Ngemboh Gresik East Java

Information index.php/JIPK/JIPK