Jurnal Ilmiah Perikanan & Kelautan
ISSN 2085-5842
Vol. 7 / No. 1 / Published : 2015-04
Order : 12, and page :71 - 78
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Original Article :
Immune response analysis of fish koi (cyprinus carpio koi) vaccinated myxobolus koi spores whole protein as a vaccine candidate myxobolusis
Author :
- Mohamad Yusuf*1
- Gunanti Mahasri*2
- Mufasirin*3
- Mahasiswa Fakultas Perairan dan Kelautan
- Dosen Fakultas Perairan dan Kelautan
- Dosen Fakultas Perairan dan Kelautan
Abstract :
Myxobolus is one of parasites on koi fish that belongs to a class myxosporea that can infect and systemic and can cause harm to the fish farming. Vaccination is an attempt to cause-specific endurance through vaccination. Observations differential leukocytes and increased optical density values can be used to determine the effectiveness of the vaccine is given.This study aims to analyze the immune response koi fish vaccinated with Myxobolus koi spores whole protein for vaccine development myxobolusis in koi fish. The method used in this study is Completely Randomized Design with 4 treatments and 5 replications.The results showed that a change in the total number and types of leukocytes that can be used as indicators of the presence of certain infectious diseases that occure in fish. The highest value of lymphocytes in treatment B, monocytes highest in treatment D, neutrophils on treatment D, eosinophils on treatment A and basophils highest in treatment A. The observation of the highest optical density value in treatment B (fish vaccinated and infected 80 M. koi spores / tail) of 0.593 at day 30, while the lowest in treatment D (fish are not vaccinated but diinveksi 80 M. koi spores / tail) of 0,064 in 30 days.
Keyword :
vaccination Myxobolus koi spore whole protein, differential leukocyte, the value of optical density, , immune response,
References :
R. Affandi,(2002) Fisiologi Hewan Air 38 - 40 : Unri Press. Pekanbaru
H. Anshary,(2008) Modul Pembelajaran Berbasis Student Center Learning (SCL) Mata Kuliah Parasitologi Ikan 126 : Jurusan Perikanan Fakultas Perikanan dan Ilmu Kelautan Universitas Hassanudin
Y. Hadiroseyani,(2003) Potensi Oligochaeta Sebagai Inang Antara Parasit Myxosporea pada Ikan Mas (Cyprinus carpio L) 37-39 : Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor
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