UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 1 / No. 1 / Published : 2009-04

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Original Article :

Pengaruh induksi bakteri pseudomonas aeruginosa terhadap human umbilical vein endothelial cells (huvecs) culture

Author :

  1. Dwi Yuni Nur Hidayati*1
  1. Fakultas Kedokteran,Universitas Brawijaya
    Jl. Veteran, Malang 65145
    Telp. 0341-560491

Abstract :

One of the cause of negative gram bacteria is Pseudomonas aeruginosa (Ps. aeruginosa). It continuing become sepsis on the first step of patogenesa happen sticking between aeruginosa with endhotel cell. This sticking is mediated by adhesi molecul which have characterize same with hemaglutin protein. The main study of this research is to know the profile of endothelial cell culture within induction of Ps. aeruginosa. The methods are Ps. aeruginosa (9064) which have been isolated then processes on TCBS Media and continued with isolation terracely. The profile of weight molecule protein obtained by SDS PAGE and then conducted electroelution. The test of hemaglutinin using eritrosit mencit. The receptor cell used is endhotel cell (HUVECs) culture. The result show that dosage of hemaglutin protein of Aeruginosa bring effect to profile of HUVECs culture determined by index adhesion of Ps. aeruginosa to HUVECs culture. We conclude that protein 38,19 kDa give effect to profile of HUVECs culture in vitro.

Keyword :

Pseudomonas aeruginosa, adhesion protein, HUVECs,


References :





Archive Article

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Volume : 1 / No. : 1 / Pub. : 2009-04
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  3. Modification Of Water Flow Rate In Tubifex Sp. Culture To Increase Quality Of Ornament Fish
  4. Effect Of L-carnitine Supplement On The Development Of Tiger Prawn Fry, Penaeus Monodon Fabr.
  5. The Utilization Of piper Betle Filtrate Crude For Curing Gurami (osphronemus Gouramy) Infected By Bacteria aeromonas Hydrophila
  6. Preliminary Study Of Cultured Hematopoietic Nila oreochromis Niloticus
  7. The Failure To Detect Taura Syndrome Virus Of Imported Shrimp penaeus Vannamei As Suspected Carrier
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