Jurnal Ilmiah Perikanan & Kelautan
ISSN 2085-5842
Vol. 1 / No. 1 / Published : 2006-03
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Original Article :
Preliminary study of cultured hematopoietic nila oreochromis niloticus
Author :
- Sudarno*1
- Laksmi Sulmartiwi*2
- Abdul Manan*3
- Woro Hastuti Satyantini*4
- Hari Suprapto*5
- Dosen Program Studi Budidaya Perairan
Fakultas Kedokteran Hewan Universitas Airlangga
Kampus C Jl. Mulyorejo – Surabaya, 60115. Telp. 031-5992785 - Program Studi Budidaya Perairan
Fakultas Kedokteran Hewan Universitas Airlangga
Kampus C Jl. Mulyorejo – Surabaya, 60115. Telp. 031-5992785 - Program Studi Budidaya Perairan
Fakultas Kedokteran Hewan Universitas Airlangga
Kampus C Jl. Mulyorejo – Surabaya, 60115. Telp. 031-5992785 - Program Studi Budidaya Perairan
Fakultas Kedokteran Hewan Universitas Airlangga
Kampus C Jl. Mulyorejo – Surabaya, 60115. Telp. 031-5992785 - Program Studi Budidaya Perairan
Fakultas Kedokteran Hewan Universitas Airlangga
Kampus C Jl. Mulyorejo – Surabaya, 60115. Telp. 031-5992785
Abstract :
The cells isolation was done from the hematopoietic tissue of nila Oreochromis niloticus.. The number of cells isolated from the hematopoietic tissue is variated, isolation was not doing well because cell could not separated perfectly. The minced of hematopoietic tissue was not perfect therefore some of cells is clumped. The cells normally culture a week and the media must be replaced with new medium for growth. The density of cell for growth is adjusted to 100000 cells per milliliter.
Keyword :
tissue culture, hematopoietic, Oreochromis niloticus,
References :
T.D. Allen,(1984) The essential cells of the hematopoietics microenvironment. - : Exp. Hematol., 12
Dexter T.M.,(1984) The essential cells of the hematopoietics microenvironment. - : Exp. Hematol., 12
B.A. Barton,(1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. - : Annual Review of Fish Diseases, 1991
Iwama G.K.,(1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. - : Annual Review of Fish Diseases, 1991
K. Blake,(1993) Cell culture. In”Virology Labfax” ed by D.R.Harper. - : Blackwell Scientific Publication
Burleson F.G.,(1992) Virology : A labora-tory manual. San Diego : Academic Press
Chambers T.M.,(1992) Virology : A labora-tory manual. San Diego : Academic Press
Wiedbrauk D.L.,(1992) Virology : A labora-tory manual. Academic Press, San Diego, pp 33-38. San Diego : Academic Press
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