ISSN 2085-5842
Vol. 7 / No. 2 / Published : 2015-01
Order : 5, and page :149 - 152
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Original Article :
Viral nervous necrosis (vnn) examination at fish by polymerase chain reaction (pcr) method
Author :
- Elly Fitriatin *1
- Abdul Manan*2
- Mahasiswa Fakultas Perairan dan Kelautan
- Dosen Fakultas Perairan dan Kelautan
Abstract :
Fish production annually has increased. Trading activities of Fishery in Indonesia both exports and imports will continue to increase every year. Control and monitoring of fisheries products was transported in Indonesia aims to reduce the risk of entry and spread of pests and diseases of fish in Indonesia. The purpose of Study is to know examination techniques of virus Viral Nervous Necrosis (VNN) at fish by Polymerase Chain Reaction (PCR) method. This Study was held in the Quarantine Fish Quality Control and Safety of Fishery Products (BBKIPM-KHP) Soekarno Hatta Tangerang, Banten on January 12th to February 12th, 2015. The methods of data fetching consisted of primary and secondary data. The data fetching was performed with active participation, observation, interviews, and literature. Examination methods Viral Nervous Necrosis (VNN) by Polymerase Chain Reaction (PCR). Stages of virus examination by PCR method includes necropsy samples, RNA extraction, amplification, electrophoresis, and the diagnosis results using UV transluminator. Fish samples were examined VNN virus consisting of a live fish and frozen fish both commodity exports, imports, or domestic. The results showed that all fish samples are negative infected with the virus VNN.
Keyword :
Viral nervous necrosis (VNN), PCR Technique, Examination result,
References :
B. Sugianti,(2005) Pemanfaatan Tumbuhan Obat Tradisional Dalam Pengendalian Penyakit Ikan hal 37 : Makalah Falsafah Sains : Institut Pertanian Bogor
L. Srigede ; S. Zaetun,(2014) Paparan Sinar Ultra Violet (UV) dengan Pengamatan Waktu Sterilisasi terhadap Pertumbuhan Bakteri Bacillus sp Vol. 8 (6) : 75-79 : Media Bina Ilmiah
Z. K.Yusuf,(2010) Polymerase Chain Reaction ((PCR) Vol.5 (6) : 1-6 : Saintek
Archive Article
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Volume : 7 / No. : 2 / Pub. : 2015-01 |
- Papaya Leaf Powder Potential To Improve Efficiency Utilization Of Feed, Protein Efficiency Ratio And Relative Growth Rate In Tilapia (oreochromis Niloticus) Fish Farming
- Characterization Of Edible Film From Propagules Mangrove Lindur (bruguiera Gymnorrhiza) Starch With Addition Of Carboxymethyl Cellulose (cmc) As Plasticizer
- Potential Of Bacillus Licheniformis And Streptomyces Olivaceoviridis As Inhibiting The Growth Of Fungus Saprolegnia Sp, Cause Saprolegniasis On Fish By Using In Vitro
- The Comparison Of Viscosity, Flash Point And Freeze Point Biodiesel Of Seagrass (eucheuma Denticulatum), Sardinella Oil (sardinellla Longiceps) And Commercial Biodiesel
- Viral Nervous Necrosis (vnn) Examination At Fish By Polymerase Chain Reaction (pcr) Method
- The Basic Histology Technique Of Gouramy Fish (osphronemus Gourami)
- The Influence Of Papaya Seed’s (carica Papaya) Toward The Damage Argulus Japonicus’s Eggs
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- Hematologic Changes Comet Goldfish (carassius Auratus Auratus) As A Result Of Infestation Argulus Japonicus Males And Argulus Japonicus Females
- Haematology And Histopatology Of Gills, Intestine And Brain Koi Fish (cyprinus Carpio Koi) Myxobolus Koi Orally Infected
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