Journal Of Marine And Coastal Science
ISSN 2301-6159
Vol. 5 / No. 1 / Published : 2016-01
Order : 1, and page :1 - 8
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Original Article :
Isolation and identification of cellulolytic bacteria from mangrove soil of gunung anyar river estuary, surabaya
Author :
- Kustiawan Tri Pursetyo*1
- Moch. Amin Alamsjah*2
- Rahayu Kusdarwati*3
- Sofie Heliza Maulani*4
- Dosen Fakultas Perairan dan Kelautan
- Dosen Fakultas Perairan dan Kelautan
- Dosen Fakultas Perairan dan Kelautan
- Mahasiswa Fakultas Perairan dan Kelautan
Abstract :
Mangroves ecosystem are kind of system arranged by two component, biotic and abiotic which have interaction in mangrove habitat. Biotic components depend on three types such as producer organism, consumer organism and decomposer organism. Mangrove litter is organic material from many decomposition processes, produce an important substance that useful for other organism and coastal productivity especially in the food chain. Mangrove litter could decompose to cellulose material by a microorganism, especially cellulolytic bacteria. The soil sample was collected at four stations from Gunung Anyar river estuary Surabaya. The soil sample that has been taking isolated in CMC agar medium, then identified by Gram test, Biochemical test and Inhibition test with a paper disc to observe capability of cellulolytic bacteria inhibit the pathogen bacteria, especially Edwardsiella tarda. Based on Gram stain Test and Biochemical Test find four species of cellulolytic bacteria such as Bacillus subtilis, Pseudomonas minute, Micrococcus luteus and Plesiomonas shigelloides. From four species that have been identifying, only Bacillus subtilis and Pseudomonas diminuta that show ability to block pathogenic bacteria Edwardsiella tarda.
Keyword :
Cellulolytic Bacteria, Mangrove, Isolation and Identification,
References :
Aydin, S., A Ciltas, H. Yetim and I. Akyurt.,(2005) Clinical, Pathologi and Haematological Effect of Micrococcus luteus in Rainbow Trout (Oncorhnycus mykiss Walbaum) 4(2): 167-174 : Journal of Animal and Veterinary Advances
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