UNIVERSITAS AIRLANGGA



Detail Article

Majalah Ilmu Faal Indonesia

ISSN 0215-1995

Vol. 11 / No. 1 / Published : 2014-03

Order : 4, and page :19 - 24

Related with : Scholar   Yahoo!   Bing

Original Article :

Curcumin inhibits carbonylation of lens protein in diabetic streptozotocin mouse models

Author :

  1. Wulandari Meilia*1
  2. Dewi Masyithoh Budi*2
  3. Setiowati Ditya Ayu Intan*3
  4. Hayuningtyas Duhita Pramesthi*4
  5. Fauzia Kartika Afrida*5
  6. Bambang Purwanto*6
  1. Mahasiswa Fakultas Kedokteran
  2. Mahasiswa Fakultas Kedokteran
  3. Mahasiswa Fakultas Kedokteran
  4. Mahasiswa Fakultas Kedokteran
  5. Mahasiswa Fakultas Kedokteran
  6. Dosen Fakultas Kedokteran

Abstract :

Lens protein carbonylation is one of mechanism that involved in complication cataract of diabetes. The aim of this study was proving the effect of curcumin antioxidant to inhibit lens protein carbonylation in diabetic mouse models. Curcumin was solved in maize (corn) oil with concentration 0.4 mL/100 mg curcumin. Diabetic mouse models were induced by intraperitoneal Streptozotocin injection with dose 150 mg/kg of body weight. Curcumin was given once a day as long as two days to the mouse that had glucose level more than 200 mg/dL. The effect of curcumin was compared to control group, aqua treated group, metformin group and curcumin+metformin group. Lens of diabetic mouse models were pounded to get homogenate that will be examined the carbonyl protein level. Absorbance was read using spectrophotometer with wave length 375 nm. Result of this research were Carbonyl protein levels (nm/ml) were like below: Control group (33.16±19.83), Diabetes+Curcumin group (32.07±9.52), Diabetes+Metformin (98.61±17.72), Diabetes+Curcumin+Metformin group (57.27±12.19), Diabetes+aquades group (311.83±73.69). Carbonyl protein level of diabetic mouse lens that given curcumin was not different with control group and curcumin+metformin group. Carbonyl protein level of diabetic mouse lens that given curcumin was significantly different with aqua treated group and metformin group. Conclusion: curcumin inhibits carbonylation of lens protein in diabetic mouse models that induced by streptozotocin. Suggestion: this experiment must be improved more in order to give option preventive therapy of cataract complication of diabetes.

Keyword :

Curcumin, Carbonyl Protein, Diabetes, Streptozotocin, Cataract,


References :

Dalle-Donne,(2008) Protein carbonyl groups as biomarkers of oxidative stress 329 (2003) 23–38 : Clinica Chimica Acta

Dayanand,(2012) Protein carbonyl content as a stable Oxidative stress marker in Type II Diabetes 3(4): 2362-2365 : Int J Biol Med Res

Jayaprakasha,(2005) Antioxidant activities of curcumin, demethoxycurcumin and bisdemethoxycurcumin 98 (2006) 720–724 : Food Chemistry





Archive Article

Cover Media Content

Volume : 11 / No. : 1 / Pub. : 2014-03
  1. Red Spinach (amaranthus Tricolor) Juice Administration To Mother Mice Exposed By Lead Acetate In Gestation Periode Improves Learning Ability And Memory Of Post Weaning Mice (mus Musculus) Offspring
  2. Effect Of Aerobic Exercise And Administration Of Mangosteen Skin Stew To Adiponectin Levels On Rats (rattus Norvegicus Wistar)
  3. Resting Heart Rate And Body Weight Are Discriminators Of Fitness Status In Physical Activity Weight Bearing Exercise
  4. Curcumin Inhibits Carbonylation Of Lens Protein In Diabetic Streptozotocin Mouse Models
  5. Submaximal-intensity Exercise And Salmon Calcitonin Improve Bone Density In Growing Rat
  6. Effect Of Physical Fitness, Physical Activity And Body Mass Index On Grade Point Average (gpa) Medical Student University Of Dublin Malahayati Bandar Lampung
  7. Effect Of King Banana Juice For Physical Exercise Performance
  8. The Influence Of Having Breakfast Towards Students’ Learning Concentration In The Morning Of Smak Santo Stanislaus Surabaya
  9. Dynamic Eccentric Activity Improving Total Glucose Uptake In Diabetes Mice Model
  10. The Quality Of Cauda Epididymal’s Spermatozoa Of Growing Ram That Feed With Ethanol Leaf Extract Of Katuk On 4ºc Storage

Information index.php/JIPK/MIFI