UNIVERSITAS AIRLANGGA



Detail Article

Majalah Ilmu Faal Indonesia

ISSN 0215-1995

Vol. 6 / No. 3 / Published : 2007-10

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Original Article :

Epigallocatechin gallate of green tea inhibits proliferation, differentiation and tnf-α in the primary human visceral preadipocytes culture

Author :

  1. Retty Ratnawati*1
  2. Rasjad Indra*2
  3. Satuman -*3
  1. -
  2. -
  3. -

Abstract :

Objectives: Green tea EGCG (epigallocathechin gallate) have shown to cause loss of body fat and also inhibit the growth of cancer cell. Therefore the aim of this study is to determine whether green tea EGCG can directly inhibit proliferation, differentiation and TNF-α (tumor necrosis factor-α) of the human visceral preadipocyte culture. Methods: Primary human visceral pre adipocytes culture were treated with EGCG in several doses 0 μM (control group), 10 μM, 50 μM, 100 μM and 200 μM for 24 hours respectively. The human visceral tissues specimens were derived from the Surgery dept. of Dr. Syaiful Anwar General Hospital in Malang. Cellular account of the adipogenesis processes (proliferation, differentiated and undifferentiated cells) was determined using microscope after hematoxylline staining. TNF-α concentration derived from primary human preadipocytes culture also observed using ELISA Kit Assay design. Results: It was found that there were a dose dependent respond to the cellular account of the adipogenesis processes. There were a significant (p=0.000) decrease of the cellular account for proliferation in EGCG control group (78.8 + 5.88%), EGCG 10 μM (66.32 + 4.63%), EGCG 50 μM (48.72+ 3.84%,), EGCG 100 μM (28.2+4.63%) and EGCG 200 μM (62.68+3.67%). There were also significant decrease of the percentages in the differentiated cells in EGCG control group (66.43+ 3.62%), EGCG 10μM (67.56+3.61%), EGCG 50 μM (62.75+2.42%), EGCG 100 μM (21.52+3.44%), EGCG 200 μM (28.04+1.24%). There were not any changes in the percentages of undifferentiation cell in the groups of EGCG 10 μM and 50 μM, however there was significant (p

Keyword :

green tea epigallocatechin gallate (EGCG), human preadipocytes, proliferation, differentiation, TNF-α ,


References :

Indra MR,(2006) Adiposit, obesitas dan masalah kesehatan global di era millenium Malang : Lab Faal FK UNIBRAW

Samad AM,(2004) Tea Extracts Inhibit the Proliferation and Differentiation of Adipocytes California : Science Fair

Aggarwal BB,(2000) Tumour necrosis factors receptor associated signaling molecules and their role in activation of apoptosis, - : JNK and NF-κB, Ann Rheum

Ahima RS,(2000) Adipose tissue as an endocrine organ - : Trends Endocrinol Metab 11

Plantenga MS,(2007) Obesity and thermogenesis related to consumption of caffeine, ephedrine, capsaicin and green tea - : Am J Physiol Regul Intege Comp Physiol

Fontaine KR,(2003) Years of life lost due to obesity. - : JAMA

Fller JS,(2000) Adipose tissue as an endocrine organ - : Trends Endocrinol Metab 11

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Archive Article

Cover Media Content

Volume : 6 / No. : 3 / Pub. : 2007-06
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  2. Perbedaan Latihan Fisik Jangka Pendek Dan Jangka Panjang Terhadap Glukosa Darah Pada Penderita Diabetes Mellitus
  3. Forced Expiratory Flow 25 Untuk Mendeteksi Kerusakan Dini Saluran Nafas Perifer
  4. Pemberian Tambahan Kalsium Pada Masa Pertumbuhan Terhadap Tebal Tulang Kortikal Dan Trabekular
  5. Physiological Aspects Of Testosterone Administration In Andropause
  6. Perbedaan Persepsi Pengecap Rasa Asin Antara Usia Subur Dan Usia Lanjut
  7. Epigallocatechin Gallate Of Green Tea Inhibits Proliferation, Differentiation And Tnf-α In The Primary Human Visceral Preadipocytes Culture

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