UNIVERSITAS AIRLANGGA



Detail Article

Majalah Ilmu Faal Indonesia

ISSN 0215-1995

Vol. 7 / No. 2 / Published : 2008-02

Order : 5, and page :101 - 105

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Original Article :

Studi shp dan protein stat pada transduksi signal hormon pertumbuhan (growth hormone) dengan teknik blotting

Author :

  1. Anwar Ma'ruf*1
  2. Nove Hidajati*2
  1. Dosen Fakultas Kedokteran Hewan
  2. Departemen Biokimia Veteriner Departemen Ilmu Kedokteran Dasar Veteriner Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya

Abstract :

The long-term objective of this study was to identify the expression pattern of SHP1 signaling protein in broiler during growth period due to increase of growth hormone (GH). This study used ten male broilers Lohman (MB 202 P) from PT. Multibreeder Indonesia Tbk. Broilers were kept within batteried cage, with a capacity of one broiler. The broilers were fed twice a day at 6 a.m. and 6 p.m. with the amount of feed 10% less than standard. At day 21 the broilers were sacrificed to obtain samples of liver for (1) isolation of SHP1 protein from liver, and muscles of the broiler, (2) analysis of SHP1 protein using SDS-PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoreses) method, and (3) identification of SHP1 protein using Western blot method by means of protein detection using electrophoresis with polyacrylamide gels. Results of examination on protein in hepatic tissue of broilers in growth period using dot blot revealed that SHP1 protein was present in those tissues. This finding was followed-up with SDS-PAGE examination, from which we found existence of protein band between the markers of 55 and 84 kDa, with molecular weight of 68 kDa. To prove that protein band formed between marker 55 and 84 kDA was the SHP1, Western blot examination was conducted using mouse monoclonal antibody SHP1. The result showed formation of the protein band, indicating presence reaction between antigen SHP1 protein and SHP1 protein antibody. In conclusion, SHP1 protein is presence in hepatic tissue, with molecular weight of 68 kDa

Keyword :

broiler, SHP1, growth hormone,


References :

Darnell J, Lodish H, Baltimora D.,(1990) Molecular cel biology New York : Scientific America Book, p 715

Endo TA, Masuhara M, Yokuuci M, et al,(1997) A new protein containing an SH2 domain that inhibits JAK kinases - : Nature 387, 921-924





Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2008-02
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  2. Penggunaan Antikanker Sari Buah Merah (pandanus Conoideus) Terhadap Kultur Sel Mieloma
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  4. Uji Spesifisitas Protein Prolaktin Dari Serum Darah Itik Molting Melalui Metode Western Blot
  5. Pengaruh Pemberian Kafein Terhadap Penghematan Glikogen Otot Pada Latihan Submaksimal
  6. Rasio Netrofil,limfosit (n Or L) Sebagai Indikator Stres Pada Owa Jawa (hylobates Moloch Audebert 1797) Di Tempat Penangkaran
  7. Potensi Capping Phenomena Dalam Analisis Asosiasi Hla Dengan Diabetes Mellitus Tipe 2
  8. Peran Endocannabinoid Dalam Pengaturan Nafsu Makan
  9. Apoptosis: Melalui Mekanismenya Menjadikan Dasar Strategi Terapi
  10. Peran Peptida Yy Pada Regulasi Homeostasis Energi
  11. Potensi Melatonin Secara Fotoneuroendokrin Sebagai Antioksidan Alamiah
  12. Studi Shp Dan Protein Stat Pada Transduksi Signal Hormon Pertumbuhan (growth Hormone) Dengan Teknik Blotting

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