UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 1 / No. 2 / Published : 2000-06

Related with : Scholar   Yahoo!   Bing

Original Article :

Efficiency of cumulus cell on culture medium in vitro one cell stage in mice embryos

Author :

  1. EM Luqman*1
  2. Widjiati*2
  3. BP Soenardirahardj*3
  4. ED Masithah*4
  5. HA Hermadi*5
  1. -
  2. -
  3. -
  4. -
  5. -

Abstract :

The aim of this research was to determine the influence of cumulus cell on in vitro culture medium toward mice embryos development from one cell until blastocyst stages. This research used two months old female and adult male mice, superovulated with PMSG and HCG. After collecting oocytes, then fertilization was carried out in vitro. After six hour zygotes were transfered to a culture medium M16, some zygotes was cultured with cumulus cell and others without cumulus cell. The result of research showed that one cell embryo that cultured without cumulus cell were better than cultured with cumulus cell. Indicating cumulus cells probably increased glucose metabolism. Early development of embryo of mice do not need much glucose after passing block development cell. Therefore in take of high glucose at early development will reduced of the develop¬ment embryo at one cell stage. The number of embryo developed until blastocyst were higher in embryo cultured without cumulus cells than cultured with cumulus cells.

Keyword :

-,


References :

Miyoshi K.,(1994) Develop¬ment of Rat One Cell Embryos in a Chemically Defined Medium; Efectsof Glucose, Phosphate and Osmolarity - : J. rep. and Fert. 100

Funahashi H,(1994) Develop¬ment of Rat One Cell Embryos in a Chemically Defined Medium; Efectsof Glucose, Phosphate and Osmolarity - : J. rep. and Fert. 100

H Saito,(1994) Cumulus Mass Maintains Embryo Quality - : J. Fert. and Sterility. Vol. 62

Y Shioya,(1988) In Vitro Fertilization and Cleavage Capability of Bovine Foli¬culer Oocyte Classified by Cumulus Cell and Matured in Vitro - : J. Teheriogenology 30

AI Younis,(1989) Influence of Serum and Hormones on Bovine Oocyte Maturation and Fertilization in Vitro - : Gamete Research 23

KM Harper,(0000) Bovine Blastocyst Development After in Vitro Maturation in A Defined Medium With Epidermal Growth Factor and Low Concentration of Gonadotropins - : Biol. Reprod. Accepted





Archive Article

Cover Media Content

Volume : 1 / No. : 2 / Pub. : 2000-06
  1. Efficiency Of Cumulus Cell On Culture Medium In Vitro One Cell Stage In Mice Embryos
  2. Penampakan Protein Darah Burung Gelatik Jawa Padda Oryzivora Berdasarkan Metode Elektroforesis
  3. The Appearence Of Blood Protein In Java Sparrow By Electrophoresis Technic
  4. The Effect Of Sulfaquuinoxaline On The Feed Consumption And Egg Production In The Lochman Brown Layer
  5. Histopatological Study Of Chicken Caecum After Being Infested With Eimeria Tenella Sporocyst
  6. Perbedaan Struktur Dan Morfologi Diantara Telur Cacing Toxocara
  7. Explorasi Bakteri Dari Uterus Sapi Infertil Di Kotamadya Surabaya
  8. Residu Zinc Bacitracin Dalam Daging, Hati Dan Ginjal Ayam Yang Diberi Feed Additive Zinc Bacitracin Selama 6 Minggu
  9. Residu Zinc Bacitracin Dalam Daging, Hati Dan Ginjal Ayam Yang Diberi Feed Additive Zinc Bacitracin Selama 6 Minggu
  10. Potensi Probiotik Dalam Meningkatkan Kecernaan Bahan Kering Pada Ayam Pedaging
  11. Kecernaan Pakan Dan Aktivitas Karbohidrase Pada Benih Guram(osphronemus Goramy Lacepede) Yang Diberi Pakan Mengandung Beberapa Jenis Karbohidrat

Information index.php/JIPK/MKH