ISSN 2015-8930
Vol. 23 / No. 3 / Published : 2007-09
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Original Article :
Marker-sets for paternity and identity test in pigs by meansmicrosatellite analysis
Author :
- Siti Darodjah Rasad*1
- Laboratorium Reproduksi Ternak, Fakultas Peternakan UNPAD
Kampus Jatinangor, Bandung 40600. Tel. 022-7798341, Fax. 022-7798212
Abstract :
The aim of this study was to establish an optimal marker set for a routine paternity and identity test. In the present work a paternity and identity test within three pig breeds (DE, DL and Pi) was performed by means of microsatellite analysis. Genotyping of the animals was done after PCR amplification of the alleles on an automated DNA sequencer (LI-COR, DNA Analyzer, GENE READIR 4200 (MWG BIOTECH). For the examination of the applicability of the microsatellite markers in paternity test, the exclusion probabilities and Polymorphism Information Contents (PICs) were calculated. Combined exclusion probability over 99 % has been achieved in all breeds by using 8 microsatellites. Fifteen of the 25 loci were selected and a group of 5 loci were packed together in three multiplex-PCRs as possible marker sets for a paternity and identity test in pig.
Keyword :
Pig, Paternity and Identity Test, Microsatellite analysis,
References :
RB Alford,(1994) Rapid and efficient resolution of parentage by amplification of short tandem repeats - : Am J Hum Genet. 55
I Alvarez,(2004) Genetic relationships and admixture among sheep breeds from Northern Spain assessed using microsatellites - : Journal of Animal Science. 82
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JC Avise,(1994) Molecular Markers. Natural History and Evolution New York : Chapman and Hall. Inc
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MM Binns,(1995) The identification of polymorphic microsatellite loci in the horse and heir use in thoroughbred parentage testing - : British Vet J. 151
D Botstein,(1980) Construction of a genetic linkage map using restriction fragment length polymorphism - : Am J Hum Genet. 32
AT Bowling,(1997) Validation of
Archive Article
| Cover Media |
Content |
Volume : 23 / No. : 3 / Pub. : 2007-03 |
- Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens
- Protein Characterization Of Snail Mucin (achasin) Local Isolate
As An Antibacterial Factor
- Trypsin And Pronase Treatment On The Development Of Mouse Embryos Contaminated By K99 Escherichia Coli
- The Optimation Of Tyrosine Kinase Activity From Sperm Isolation Of Friesian Holstein Cow
- Insulin-like Growth Factor I (igf-i) Complex Seminal Plasma Of Goatratio Glicoprotein : Protein : Carbohydrate
- The Role Of Choline Esterase On Vesicle Brain Development Of Chicken Embryo Of Exposure By Carbofuran Insecticide
- Marker-sets For Paternity And Identity Test In Pigs By Meansmicrosatellite Analysis
- Bcg Vaccination Increased Roi Secretion In Dogs Macrophage Infected With Mycobacterium Tuberculosis
- Antifertility Effect Of Aqoueus Extract Betel Nut Of Areca Catechu: As An Apoptosis Agent On Testis Connective Cells Of Rattus Norvegicus
- The Morphology Of Mandibular And Lingual Glands Of Chicken (gallus Sp) And Quail (coturnix Coturnix): With Special Refference To The Distributionand Carbohydrate Content
- Detection Of Rabies Virus In Saliva And Brain Using Antibody From G Protein As Material Diagnostic By Indirect Double Antibody Sandwich Elisa Technique
- Study To Rice Brand In Drying Ensilage Product From Edible Snail For Decrease Higroscopies As Feed Stuff
- Potency Rice Straw Was Deammoniated And Fermented By Celulolytic Bacteria On The Dry Matter Consumption, Body Weight Gain And Feed Conversion Of Sheep
- Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens
- Protein Characterization Of Snail Mucin (achasin) Local Isolate
As An Antibacterial Factor
- The Role Of Choline Esterase On Vesicle Brain Development Of Chicken Embryo Of Exposure By Carbofuran Insecticide
- The Optimation Of Tyrosine Kinase Activity From Sperm Isolation Of Friesian Holstein Cow
- Insulin-like Growth Factor I (igf-i) Complex Seminal Plasma Of Goat
Ratio Glicoprotein : Protein : Carbohydrate
- Marker-sets For Paternity And Identity Test In Pigs By Means Microsatellite Analysis
- Bcg Vaccination Increased Roi Secretion In Dogs Macrophage Infected With Mycobacterium Tuberculosis
- Antifertility Effect Of Aqoueus Extract Betel Nut Of Areca Catechu: As An Apoptosis Agent On Testis Connective Cells Of Rattus Norvegicus
- Trypsin And Pronase Treatment On The Development Of Mouse Embryos
Contaminated By K99 Escherichia Coli
- The Morphology Of Mandibular And Lingual Glands Of Chicken (gallus Sp) And Quail (coturnix Coturnix): With Special Refference To The Distribution And Carbohydrate Content
- Detection Of Rabies Virus In Saliva And Brain Using Antibody From G Protein As Material Diagnostic By Indirect Double Antibody Sandwich Elisa Technique
- Study To Rice Brand In Drying Ensilage Product From Edible Snail For Decrease Higroscopies As Feed Stuff
- Potency Rice Straw Was Deammoniated And Fermented By Celulolytic Bacteria On The Dry Matter Consumption, Body Weight Gain And Feed Conversion Of Sheep
- Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens
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