Media Kedokteran Hewan
ISSN 2015-8930
Vol. 17 / No. 3 / Published : 2001-12
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Original Article :
Pregnancy rate and sex ratio offspring resulted from separation of y spermatozoa with column albumin methods
Author :
- Erma Safitri*1
- Herry Agoes Hermadi*2
- Abdul Samik*3
- Lab. Ilmu Kemajiran Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya.
- Lab. Ilmu Kemajiran Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya.
- Lab. Fisiologi Reproduksi Fakultas Kedokteran Hewan, Universitas Airlangga
Abstract :
The aim of this research was to know the result of X and Y sperm separated by using column albumin toward pregnancies rate and sex offspring ratio of goats. The research was used 12 goats consist of 10 does and 2 bucks. The 10 does divided into 2 groups of control (group I) and experiment group (group II). The Oestrus were synchronized by using PGF2 and PMSG before artificial insemination. Each goat in the group I was inseminated with fresh semen which was diluted by fresh milk as 1:9, group II was inseminated with fresh semen resulted from column albumin methods of separation. Semen were taken from bucks and observed of semen quality. Sperm concentration were 2,69 x 109/ ml, 82,75% of motility and 9,75% of abnormality. After separation of sperm by column albumin methods, motility of spermatozoa were 76,50% and 20,75% of abnormality. The result showed that pregnancy rate were 80% (group I) and 60% (group II). Total offspring were 4 (44%) male and 5 (56%) female in group I and 5 (71%) male, 2 (29%) female in group II. Separation of spermatozoa by column albumin showed that weight of Y spermatozoa was less than X spermatozoa. Pregnancy rate depends on some factors, including quality of semen (concentration, motility, abnormality), fertility of does, semen handling, time and technique of artificial insemination.
Keyword :
Semen, pemisahan spermatozoa X dan Y, inseminasi buatan, sex ratio anak,
References :
Archive Article
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