UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 17 / No. 3 / Published : 2001-12

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Original Article :

The effect of sperm treatments of madura cattle on live percentage, motility and cleavage rate

Author :

  1. Imam Mustofa*1
  2. Laba Mahaputra*2
  1. Laboratorium Kebidanan Veteriner Fakultas Kedokteran Hewan Universitas Airlangga
  2. Laboratorium Kebidanan Veteriner Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

This research was conducted to investigate the effect of separation X and Y bearing chromosome spermatozoa using several techniques on the percentage of live and motility of sperm, and cleveage rate after in vitro fertilization using its sperm. Frozen thawed Madura bull semen were treated in six different methods to separate X and Y bearing chromosome spermatozoa by using sephadex, percoll, Swim up, Swim up + aside migration, electric current 1.5 Volt and control group. Ovaries were collected from an abattoir and subsequently oocytes were matured in a TCM199 media with a supplement of 10% estrous cow serum in a 5% C02 incubator for 24 hours. Forty eight hours after the in vitro fertilization using the sperm collected from these separation methods, the percentage of cleavage was determined. The result showed that the percentage of live sperm after treatment using Sephadex and Percoll column were not significantly different (p>0.05) compared to control group. The percentage of motil sperm was similar (p>0.05) or decreased (p

Keyword :

X Y sperm separation, Madura cattle.,


References :





Archive Article

Cover Media Content

Volume : 17 / No. : 3 / Pub. : 2001-12
  1. The Effect Of Acepromazine Premedication On Intraocular Pressure In Dogs Anasthetized With Ketamine Hcl
  2. The Effect Multi Dosage Of Sulphadiazine On Histopathological Liver Of Mice (mus Musculus)
  3. Preliminary Study On The Isolation And Identification Of Yeasts In Horse Milks Distributed In Bandung Region
  4. Pregnancy Rate And Sex Ratio Offspring Resulted From Separation Of Y Spermatozoa With Column Albumin Methods
  5. Extremely Low Frequency Electromagnetic Field Effects On The Histologic Properties Of Rat Aorta
  6. The Number Trypanosoma Evansi In Mice Blood During The Acute And Chronic Phases
  7. Pregnancy Rate And Sex Ratio Offspring Resulted From Separation Of Y Spermatozoa With Column Albumin Methods
  8. The Use Of Progesterone Levels In Miik For Determination The Accuracy Of Timing Of Insemination In Dairy Cows
  9. The Effect Of Sperm Treatments Of Madura Cattle On Live Percentage, Motility And Cleavage Rate
  10. Follicle Size Selection Influences Of Chromosome Transformation Profile On Goat Oocytes In In-vitro Maturation
  11. The Effect Of Treatment Of Prophyltiouracyl On Male Rat Against Pregnancy Of White Female Rat

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