UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 23 / No. 3 / Published : 2007-09

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Original Article :

Trypsin and pronase treatment on the development of mouse embryos contaminated by k99 escherichia coli

Author :

  1. I Wayan Batan*1
  2. Arief Boediono*2
  3. Ita Djuwita*3
  4. Bibiana Widiati Lay*4
  5. Supar*5
  1. Bagian Klinik Hewan Fakultas Kedokteran Hewan Universitas Udayana,
    Jalan Raya Sesetan Gang Markisa 6 Denpasar Bali 80232. Telp./Fax. 0361-8423062
  2. Dept Anatomi Fisiologi dan Farmakologi,FKH IPB, Jl. Agatis Kampus IPB Dramaga Bogor 16680
  3. Dept Anatomi Fisiologi dan Farmakologi,FKH IPB, Jl. Agatis Kampus IPB Dramaga Bogor 16680
  4. Dept Ilmu Penyakit Hewan FKH IPB,
    Jl. Agatis Kampus IPB Dramaga Bogor 16680
  5. Balai Besar Penelitian Veteriner Jl. Martadinata No 30, Bogor 1611

Abstract :

The objectives of this studies were to investigate the effect of washing on development of embryos wich experimentally exposed with K99 E.coli. The embryos were exposed to K99 E.coli in appropriate amount of 103 CFU/ml and then incubated at 370C for an hour, then they were devided into three groups. The first group were washed with mPBS and KSOM, the second group of embryo were treated with trypsin for 90 second, the third group were treated with pronase for 60 second. Each group of the embryos were washed 6 times in mPBS and 4 times in KSOM, Thereafter, the embryos were cultured in the drops of KSOM without antibiotic and incubated in incubator 5% CO2 at 370C. The development embryos were observed every six hours interval for 48 hours period. The result of this studies showed that the development of pronase treated embryos were better than that of the mPBS washing and the trypsin treated. In conclussion of the present studies show that the development of embryos could be influenced by the K99 E.coli contaminated medium. Trypsin and pronase treatment could minimize the negative effect of K99 E.coli on embryos under in vitro conditions.

Keyword :

embryos, K99 E.coli, contaminated, trypsin, and pronase ,


References :

IW Batan,(2006) Pelacakan perlekatan bakteri Escherichia coli K99 pada zona pellucida embrio mencit dengan metode enzym linked immunosorbent assay (ELISA) dan scanning electron microscope (SEM - : J. Veteriner. 7

A Bielanski,(1997) A review on disease transmission studies in relationship to production of embryos by in vitro fertilization and to related new reproductive technology - : Biotech Advance 15

A Bielanski,(2000) Effect of Mycoplasma bovis and Mycoplasma bovigenitalium in Semen on fertlization and association with in vitro produced morula and blastocyst stage embryo - : Theriogenology. 53

A Bielanski,(2004) Observation on the fertilization and development of preimplantation bovine embryos in vitro in the p0esence of Trichomonas foetus - : Theriogenology. 61

A Bielanski,(1996) Washing or washing and trypsin treatment is ineffective for removal of noncytopathic bovine viral diarrhea virus from bovine oocytes or embryos after experimental viral contam



Archive Article

Cover Media Content

Volume : 23 / No. : 3 / Pub. : 2007-03
  1. Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens
  2. Protein Characterization Of Snail Mucin (achasin) Local Isolate As An Antibacterial Factor
  3. Trypsin And Pronase Treatment On The Development Of Mouse Embryos Contaminated By K99 Escherichia Coli
  4. The Optimation Of Tyrosine Kinase Activity From Sperm Isolation Of Friesian Holstein Cow
  5. Insulin-like Growth Factor I (igf-i) Complex Seminal Plasma Of Goatratio Glicoprotein : Protein : Carbohydrate
  6. The Role Of Choline Esterase On Vesicle Brain Development Of Chicken Embryo Of Exposure By Carbofuran Insecticide
  7. Marker-sets For Paternity And Identity Test In Pigs By Meansmicrosatellite Analysis
  8. Bcg Vaccination Increased Roi Secretion In Dogs Macrophage Infected With Mycobacterium Tuberculosis
  9. Antifertility Effect Of Aqoueus Extract Betel Nut Of Areca Catechu: As An Apoptosis Agent On Testis Connective Cells Of Rattus Norvegicus
  10. The Morphology Of Mandibular And Lingual Glands Of Chicken (gallus Sp) And Quail (coturnix Coturnix): With Special Refference To The Distributionand Carbohydrate Content
  11. Detection Of Rabies Virus In Saliva And Brain Using Antibody From G Protein As Material Diagnostic By Indirect Double Antibody Sandwich Elisa Technique
  12. Study To Rice Brand In Drying Ensilage Product From Edible Snail For Decrease Higroscopies As Feed Stuff
  13. Potency Rice Straw Was Deammoniated And Fermented By Celulolytic Bacteria On The Dry Matter Consumption, Body Weight Gain And Feed Conversion Of Sheep
  14. Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens
  15. Protein Characterization Of Snail Mucin (achasin) Local Isolate As An Antibacterial Factor
  16. The Role Of Choline Esterase On Vesicle Brain Development Of Chicken Embryo Of Exposure By Carbofuran Insecticide
  17. The Optimation Of Tyrosine Kinase Activity From Sperm Isolation Of Friesian Holstein Cow
  18. Insulin-like Growth Factor I (igf-i) Complex Seminal Plasma Of Goat Ratio Glicoprotein : Protein : Carbohydrate
  19. Marker-sets For Paternity And Identity Test In Pigs By Means Microsatellite Analysis
  20. Bcg Vaccination Increased Roi Secretion In Dogs Macrophage Infected With Mycobacterium Tuberculosis
  21. Antifertility Effect Of Aqoueus Extract Betel Nut Of Areca Catechu: As An Apoptosis Agent On Testis Connective Cells Of Rattus Norvegicus
  22. Trypsin And Pronase Treatment On The Development Of Mouse Embryos Contaminated By K99 Escherichia Coli
  23. The Morphology Of Mandibular And Lingual Glands Of Chicken (gallus Sp) And Quail (coturnix Coturnix): With Special Refference To The Distribution And Carbohydrate Content
  24. Detection Of Rabies Virus In Saliva And Brain Using Antibody From G Protein As Material Diagnostic By Indirect Double Antibody Sandwich Elisa Technique
  25. Study To Rice Brand In Drying Ensilage Product From Edible Snail For Decrease Higroscopies As Feed Stuff
  26. Potency Rice Straw Was Deammoniated And Fermented By Celulolytic Bacteria On The Dry Matter Consumption, Body Weight Gain And Feed Conversion Of Sheep
  27. Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens

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