UNIVERSITAS AIRLANGGA



Detail Article

Journal Orthopaedi and Traumatology Surabaya

ISSN 2460-8742

Vol. 7 / No. 1 / Published : 2018-04

Order : 5, and page :42 - 54

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Original Article :

The effect of decellularization technique on collagen type ii and matrices porosity of cartilage bovine scaffold

Author :

  1. Dwikora Novembri Utomo*1
  2. Ika Benny Kartika Sari *2
  1. Dosen Fakultas Kedokteran
  2. Mahasiswa Fakultas Kedokteran

Abstract :

Background: Cartilage has limited capacity for self-repair in case of defect. Stem cell based tissue engineering has become an alternative therapy for the treatment of cartilage defect. The use of decellularized cartilage bovine can serve as scaffold to support proliferation dan differentiation of the stem cell. Purpose: This research is to compare the effect of decellularization technique on collagen type II and matrices porosity of cartilage bovine scaffold. Methods: This experimental laboratory research used cartilage bovine scaffold which was decellularized using physical, chemical and enzymatic techniques with different time and concentration variations, then collagen type II measurement was performed by immunohistochemical method and evaluation of porosity size with electron microscopy. Results: In the preparations with the highest concentration and the longest immersion time of 5% SDS for 72 hours, the decellularization process occurs perfectly. The collagen type II content was found to have significantly higher concentrations than the controls and other preparations, and have interconnected porosities in large size. Conclusion: High concentrations of collagen type II  and large diameter of porus after decellularization process in cartilage bovine scaffold confirm that this scaffold still has optimum biochemical cues that can be a good mechanical framework and microenvironment stem cell in effort to repair the cartilage defects. This result was expected to become a standard for the deselularization making of cartilage bovine scaffold.

Keyword :

cartilage joint, cartilage bovine scaffold, decellularization,


References :

Asheesh Bedi, Brian T. Feeley, Riley J. Williams,(2010) Management of Articular Cartilage Defects ofthe Knee 92:994-1009 : Journal of Bone and Joint Surgery, Am

Ferdiansyah,(2007) Use of Freeze-Dried Irradiated Bones in Orthopaedic Surgery.In Radiation in Tissue Banking Basic Science and Clinical Applications of Radiated Tissue Allografts 317-326 : World Scientific, Singapore

Konakci KZ, Bohle B, Blumer R, Hoetzenecker W, Roth G, Moser B, et al.,(2005) Alpha-Gal on bioprostheses: xenograft immune response in cardiac surgery 35:17–23 : Eur J Clin Invest





Archive Article

Cover Media Content

Volume : 7 / No. : 1 / Pub. : 2018-04
  1. Correlation Between Three Column Concept Classification And Internal Fixation Procedure Of Tibial Plateau Patients At Dr. Soetomo Hospital
  2. Correlation Between Harris Hip Score (hhs) And Body Mass Index (bmi) In Patients With Femoral Neck Fracture After Hemiarthroplasty
  3. Osteosarcoma Has Not Become Attention To Society Profile Of Osteosarcoma Patients At Dr. Soetomo General Hospital Surabaya “a Retrospective Study”
  4. The Role Of Platelet-rich Fibrin Facilitates The Healing Of Gastrocnemius Muscle Defect: A Preliminary Study On Animal Model
  5. The Effect Of Decellularization Technique On Collagen Type Ii And Matrices Porosity Of Cartilage Bovine Scaffold
  6. Long Term Functional Evaluation Autofibular Strut Graft In Case Of Fractures Neck Femur In Rsud Dr. Soetomo Surabaya
  7. Biomechanical Comparative Study Between Tension Band Wiring, Double Endo Button And Hook Plate For Acromioclavicular Joint Injury
  8. Orthopaedic Emergency Cases At Hasan Sadikin Hospital Bandung Caused By Traditional Bone Setter Practice
  9. Lower Leg Deformity Correction Using Ilizarov Device In Dr Soetomo General Hospital Surabaya: A Case Series

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