Journal Orthopaedi and Traumatology Surabaya
ISSN 2460-8742
Vol. 1 / No. 1 / Published : 2012-01
Order : 3, and page :16 - 20
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Original Article :
The effect of n-acetylcysteine (nac) on tissue malondialdehyde (mda) level and tissue edema in ischemia reperfusion injury of rat (rattus norvegicus) skeletal muscle
Author :
- Didyn Nuzul Ariefin*1
- Erwin Ramawan*2
- Resident Orthopedi dan Traumatologi Surabaya
- Konsultan senior Departemen Orthopedi dan Traumatologi
Abstract :
Background: Generation of free radical is one of central mechanisms in ischemia reperfusion injury (IRI) . It exerts deleterious effect on cell membrane through lipid peroxidation. N-acetylcysteine (NAC), an antioxidant, that may improve microcirculation and attenuate neutrophil activation.Objectives : The aim of this study was to scrutinize any possible protective effect of NAC in ischemia reperfusion injury of skeletal muscle by measuring tissue malondialdehyde (MDA) and tissue edema, and to explore whether the effect was dose dependant or not. Pentoxifylline was also tested for comparisonMethods : Acute hind limb ischemia in rats was induced for 4 h through vascular ligation and torniquet application and followed by 1 h reperfusion. Gastrocnemius muscle samples were obtained at the end of reperfusion. Muscle injury was evaluated in 5 experimental groups: 1) control (saline), 2) Pentoxifylline 20 mg/kg, 3) NAC 200 mg/kg, 4) NAC 400 mg/kg, 5) 800 mg/kg.Results : NAC groups had significantly lower muscle tissue MDA level as compared to the control group (p<0,05). There were no significant differences of muscle tissue MDA level among NAC 200, 400, 800 groups, and also between NAC and Pentoxifyylline group (p>0,05). There were no significant differences of tissue water content among experimental groups (p>0,05).Conclusion : NAC was found to attenuate lipid peroxidation associated membrane injury in this IRI model of limb ischemia. NAC 200 mg/kg was the recommanded dose. It was needed further investigation with longer follow up period and other parameters of IRI to study the protective effects of NAC.
Keyword :
Ischemia reperfusion injury, N-Acetylcysteine, lipid peroxidation, MDA, tissue edema,
References :
Carden DL, Granger DN,(2000) Pathophysiology of ischaemia-reperfusion injury - : J Pathol
Carlson RE, Aisen AM, Buda AJ,(1992) Effect of reduction in myocardial edema on myocardial blood flow and ventricular function after coronary reperfusion - : Am J Phvsiol
Florian Dick, Jianhui Li, Marie-Noëlle Giraud, Christoph Kalka, Juerg Schmidli and Hendrik Tevaeara,(2008) Basic Control of Reperfusion Effectively Protects Against Reperfusion Injury in a Realistic Rodent Model of Acute Limb Ischemia - : Circulation
Sahin, S., Karabey, Y., Kaynak, M.S., Hincal, A.A,(2006) Potential use of freeze-drying technique for estimation of tissue water content - : Methods Find Exp Clin Pharmacol
Kerksick C and Willoughby D,(2005) The Antioxidant Role of Glutathione and N-Acetyl-Cysteine Sulements and Exercise-Induced Oxidative Stress - : J Int Soc Sports Nutr
Archive Article
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 Volume : 1 / No. : 1 / Pub. : 2012-11 |
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