UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 3 / No. 2 / Published : 2014-10

Order : 12, and page :281 - 285

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Original Article :

Identification fertility associated antigen (faa) in seminal vesicle of brahman bull using sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) and western blot

Author :

  1. Ruswita Permana Sari*1
  2. Anwar Ma’ruf*2
  3. Rr. Sri Pantja Madyawati*3
  4. Tri Wahyu Suprayogi*4
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

Fertility Associated Antigen was a protein which had a function as a facilitator in the capacitation of spermatozoa during ejaculation. The presence of this in membrane plasma spermatozoa and plasma seminalis could increase the fertility of the bull. The aim of this study was to identify Fertility Associated Antigen (FAA) in the accessory sex glands of bull (the source of seminal plasma). This research used accessory sex glands from nine bulls. It was obtained from slaughterhouse in Ketanon, Tulungagung, extracted using muller mortar and mixed with PBS. That samples were identificated using the SDS-PAGE and Western Blot technique for more specific result. The result from SDS PAGE showed 9 protein bands, namely: 181 kDa, 73,53 kDa, 66,33 kDa, 54,79 kDa, 44,40 kDa, 35,76 kDa, 30,22 kDa, 24,79 kDa and 19,12 kDa. From that result here were several protein bands of proteins with molecular weight of 30,22 kDa which identified as Fertility Associated Antigen (FAA) after being tested by monoclonal antibody anti-FAA. The conclusion from this research was that Fertility Associated Antigen (FAA) could be identified on accessory sex glands of bull.

Keyword :

Accessory Sex Gland, bull, FAA, SDS-PAGE, Western Blot,


References :

Bellin, M.E., J.N. Oyarzo, H.E. Hawkins, H. Zhang, R.G. Smith, D.W. Forrest, L.R. Sprott, and R.L. A,(1998) Fertility Associated Antigen on Bull Sperm Indicates Fertility Potential. USA : J Anim Sci, 76:2032-2039.

Harmini, R.W. Asmarantaka, dan J. Atmakusuma. ,(2011) Model Dinamis Sistem Ketersediaan Daging Sapi Nasional. Indonesia : Jurnal Ekon Pemb. 12 (1) :128-146.

Mccauley, T. D., H. Zhang, M.E. Bellin, dan R.L. AX. ,(1999) Purification and Characterization of Fertility Associated Antigen (FAA) in Bovine Seminal Fluid. USA : Molec. Reprod and Develop. 54:145–153

Noyes, J. H., K. Bruce, Johnson, D. Larry, Bryant, L. Scott, Findholt, J. W. Thomas. ,(2011) Effects of Bull Age on Conception Dates and Pregnancy Rates of Cow Elk. USA : J Wildl Manage, 60 (3) : 508-517

Sprott, L. R., M.D. Harris, D.W. Forrest, J. Young, H.M. Zhang. J.M. Oyarzo, M.E. Bellin, and R.L. A,(2000) Artificial Insemination Outcomes in Beef Females Using Bovine Sperm with a Detec



Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-10
  1. Measurement Of Nuclear Dna Damage Of Simental Bull Spermatozoa Post Thawing With Sentrifuged Egg Yolk Skim And Soybean Lecitin Diluter
  2. Viability Of Spermatozoa Merino Sheep With Addition Of Various Nacl Concentration
  3. Numbers Resistance Spermatozoa Of Merino Rams And Etawa Goat In Nacl 0.9%, Nacl 1% And Nacl 1.1%
  4. Response To The Onset Of Estrous After Revocation Mpa (medroxy Progesterone Acetate) Intravaginal With Followed By Giving Gonadotropin Hormone On Fat Tailed Sheep
  5. Effect Of Pregnant Mare Serum Gonadotropin (pmsg) In Combination With Follicle Stimulating Hormone (fsh) For Superovulatory Treatment On The Onset And Duration Of Estrus In Fat Tailed Ewes (ovis Aries)
  6. The Effect Of Various Thawing Time On Frozen Semen Of Fat Tailed Sheep For Viability, Motility And Intact Plasma Membrane Of Spermatozoa
  7. Efforts To The Revenue Livestock Laying Duck And Fight Through The Surface Water Pollution Feed Management, Waste And Diversified Business
  8. Reproduction Efficiency Of Artificial Insemination Beef Cattle In East And West District, Kabupaten East Lombok On 2012
  9. Reproductive Efficiency Of Limousin And Simmental Cross Resulted From Artificial Insemination (ai) During 2012 In Ngoro, Jombang
  10. The Influence Of Mangosteen Peels Infusion (garcinia Mangostana L.) To Acrosome Reaction On Rats Spermatozoa
  11. Addition Of Cattle Seminal Plasma To Sperm Quality Goat On Freezing Process With Time Equilibration Different
  12. Identification Fertility Associated Antigen (faa) In Seminal Vesicle Of Brahman Bull Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (sds-page) And Western Blot
  13. Isolation And Identification Of Osteopontin Sperm Membrane An Effort To Improve The Quality Of Holstein Fressian Dairy Bull Frozen Semen
  14. Suplementation Of Insuline-like Growth Factor-i Pregnant Thoroughbred Mare Serumon Increase Of Bovine Embrio Cleavage Rates

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