UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 6 / No. 1 / Published : 2017-04

Order : 10, and page :45 - 49

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Original Article :

Pengaruh pemberian insulin-like growth factor-i (igf -i) dari serum kuda crossbreed bunting terhadap siklus estrus mencit (mus musculus)

Author :

  1. Putri Sulisdiani*1
  2. Dr. Tjuk Imam Restiadi, drh., M.Si.*2
  3. Roesno Darsono, drh., M.Vet.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The purpose of the research was to determine the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) on estrous cycle in mice (Mus musculus). The subject of this research were 35 female mice. The research was arranged by Completely Randomized Design (CRD) with seven treatment and five replications. The treatment were K0 = 10 ng/ml of physiological NaCl, P11 = 10 ng/ml of IGF-I PMS, P12 = 20 ng/ml of IGF-I PMS, P13 = 40 ng/ml of IGF-I PMS, P21 = 10 ng/ml of IGF-I recombinant mouse, P22 = 20 ng/ml of IGF-I recombinant mouse, and P23 = 40 ng/ml of IGF-I recombinant mouse. Observed variables include estrous cycle phases that proestrus, estrus, metestrus, and diestrus phase. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS did not significantly affect (p>0,05) on proestrus, metestrus, and diestrus phase. The addition of IGF-I PMS 40 ng/ml was extended (p<0,05) the estrus phase. It showed that did not significantly difference (p>0,05) between the effect of IGF-I PMS and IGF-I recombinant mouse against the mice estrous cycles.

Keyword :

IGF-I, IGF-I pregnant crossbreed mare serum, estrous cycle, Mus musculus,


References :

Inoue, Ayako., Takeuchi, Sakae, and T. Sumio.,(2005) Insulin-Like Growth Factor-I Stimulated DNA Replication in Mouse Endometrial Stroma Cells. 51 : 3. : Journal of Reproduction and Development.

Nalley, W.M.M., Handarini, R., Rizal, M., Arifiantini, R.I., Yusuf T.L., dan B. Purwantara.,(2011) Determination of The Estrous Cycle Based on Vaginal Cytology and Hormone Profile in Timor Hind. 12(2) : 98-106. : Jurnal Veteriner.

Pine, MD., Hiney, J.K., Dearth, R.K., Brattonk, G.R. and Dees WL.,(2005) IGF-I Administration to Prepubertal Female Rats Can Overcome Delayed Puberty Caused by Maternal Pb Exposure. 21 (1) : 104 - 109. : Reprod Toxicol.

Sutasurya, L.A., Sitasiwi, A.J and Ratnawati K.,(2001) Penentuan Kandungan Estradiol (E2) dan Luteinizing Hormone (LH) pada Petaurus Breviceps Papuanus (Marsupialia) Selama Satu Siklus Estrus. 11 : 25-35. : Jurnal Sains dan Teknologi Nuklir Indonesia.





Archive Article

Cover Media Content

Volume : 6 / No. : 1 / Pub. : 2017-04
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  2. Penerapan Chaid Sebagai Alat Pengklasifikasi : Studi Kasus Tanggap Peternak Pada Pengabdian Masyarakat Tentang Inseminasi Buatan Pada Sapi Madrasin Di Desa Sembilangan Kabupaten Bangkalan Madura
  3. Pengaruh Penambahan Glukosa Sebagai Sumber Energi Terhadap Viabilitas Dan Motilitas Spermatozoa Sapi Madura Dalam Pengencer Susu Skim Kuning Telur
  4. Pengaruh Pemberian Ekstrak Buah Terong Ungu (solanum Melongena L.) Pada Mencit (mus Musculus) Terhadap Angka Kebuntingan
  5. Conception Rate Dan Service Per Conception Pada Sapi Potong Hasil Inseminasi Buatan Di Kecamatan Sungai Raya Dan Kecamatan Teluk Pakedai Kabupaten Kubu Raya Provinsi Kalimantan Barat Tahun 2014
  6. Gambaran Histopatologi Epididimis Kelinci (oryctolagus Cuniculus) Setelah Dilakukan Imunisasi Dengan Vaksin Whole Bakteri Brucella Abortus Strain 19
  7. Pola Resistensi Antibiotik β-laktam Pada Escherichia Coli Yang Diisolasi Dari Susu Di Peternakan Sapi Perah Surabaya
  8. Pola Resistensi Antibiotik Non Beta Laktam Pada Escherichia Coli Yang Diisolasi Dari Susu Di Peternakan Sapi Perah Surabaya
  9. Perbandingan Kualitas Susu Antara Pasar Dan Kud Argopuro Krucil, Probolinggo
  10. Pengaruh Pemberian Insulin-like Growth Factor-i (igf -i) Dari Serum Kuda Crossbreed Bunting Terhadap Siklus Estrus Mencit (mus Musculus)
  11. Pengaruh Penambahan Plasma Seminalis Sapi Simmental Terhadap Motilitas Dan Viabilitas Spermatozoa Domba Ekor Gemuk Setelah Ekuilibrasi Pada Proses Pembekuan
  12. Pengaruh Musim Terhadap Conception Rate Dan Service Per Conception Sapi Potong Akseptor Inseminasi Buatan Di Kabupaten Nganjuk
  13. Efisiensi Reproduksi Dan Indeks Fertilitas Sapi Perah Friesian Holstein Hasil Inseminasi Buatan Pada Berbagai Paritas Dan Umur Di Wilayah Kud Tani Wilis, Sendang, Tulungagung Periode Tahun 2014
  14. Pengaruh Pemberian Serbuk Buah Terong Ungu (solanum Melongena L) Terhadap Proses Spermatocytogenesis Testis Tikus Putih (rattus Norvegicus) Pasca Pemberian Diet Tinggi Lemak
  15. Mutasi Dan Delesi Pada Genotip Sapi Madura Akibat Persilangan

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