UNIVERSITAS AIRLANGGA



Detail Article

Planta Husada

ISSN 2338-7130

Vol. 2 / No. 1 / Published : 2014-04

Order : 2, and page :5 - 11

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Original Article :

Ethyl acetate fraction of andrographis paniculata nees increases cytotoxic effect of vincristine on human cancer cell lines

Author :

  1. Sukardiman*, Mulja Hadi Santosa, Lusiana Arifianti and Frinka Martha R *1
  2. Sukardiman*2
  3. Mulja Hadi Santosa*3
  4. Lusiana Arifianti *4
  5. Frinka Martha R *5
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Dosen Fakultas Farmasi
  4. Dosen Fakultas Farmasi
  5. Mahasiswa Fakultas Farmasi

Abstract :

Objective: The objective of the present investigation was to examine  whether ethyl acetate fraction from Andrographis paniculata Nees (EAA) synergizes the therapeutic potential of  vincristine (VCR) against different human cancer cell lines (T47D, HeLa, and WiDr). Materials and Methods: MTT assay was used to measure the growth inhibitory effect of the  combination. Synergistic efficacy was subjected to median effect analysis with nonexclusive model as previously described by Chou and Talalay. Result: IC50 of EAA were 18.486 µg/ml in HeLa cells line, 13.467 µg/ml in Widr cells line and 21.618 µg/ml in T47D cells line. EAA and VCR combined as a cocktail, synergistically inhibited the growth of cancer cells in vitro, with Combination Index value (CI) ranging from 0.975 to 0.224 in T47D cells line, 0.447 in HeLa cells line and 0.887 to 0.326 in WiDr cells lines. Conclusion: EAA and VCR, combined as a cocktail, showed synergism in inhibiting the growth of human breast cancer  cells (T47D) in vitro.

Keyword :

Andrographis paniculata Nees, , Vincristine, Ethyl Acetate Fraction, Human Cancer Cell Lines,


References :

Trevor AJ., Katzung BG, Masters S, ,(2007) Cancer Chemotherapy New York : McGraw-Hill Companies, Inc.

Sukardiman-Harjotaruno, Aty-Widyawaruyanti, Sismindari, Noor-Cholies-Zaini,(2007) Apoptosis Inducing Effect of Andrographolide on TD-47 Human Breast Cancer Cell Line - : African Journal Traditional Complementary Alternative Medicine

Kumar RA, Sridevi K., Kumar NV,(2004) Anticancer and immunostimulatory compounds from Andrographis paniculata. - : J Ethnopharmacology

Matsuda T, Kuroyanagi M, Sugiyama S, Umehara K, Ueno A, Nishi K, ,(1994) Cell-differentiation-inducing diterpenes from Andrographis paniculata - : Chem Pharm Bull

Lei XY, Kong L, Su XY, Guo M, Zou HF,(2008) Biological fingerprinting analysis of interaction between taxoids in Taxus and microtubule protein by microdialysis coupled, with high-performance liquid chromatography/mass spectrometry for screening antimicrotubule agents - : Chem Res Chinese U





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2014-04
  1. Eluent Effect On Sinensetin In Orthosiphon Stamineus Benth. Leaves Extract
  2. Ethyl Acetate Fraction Of Andrographis Paniculata Nees Increases Cytotoxic Effect Of Vincristine On Human Cancer Cell Lines
  3. Antiangiogenesis From Pericarp Of Mangosteen On T47d Breast Cancer
  4. Ethyl Acetate Fraction Of Johar Leaves (cassia Siamea Lamk) On Plasmodium Falciparum Strain G2300 (chloroquin Resistence) In Vitro As An Antimalaria Activity
  5. Pinostrobin Isolated From Kaempferia Pandurata Roxb Induced Apoptosis In T47d Human Beast Cancer Cell Line

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