UNIVERSITAS AIRLANGGA



Detail Article

PharmaScientia

ISSN 2302-0725

Vol. 2 / No. 1 / Published : 2013-07

Order : 3, and page :21 - 30

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Original Article :

Optimasi mikrosfer ovalbumin-alginat yang diproduksi dengan teknik aerosolisasi

Author :

  1. Dewi Melani Hariyadi*1
  2. Esti Hendradi*2
  3. Octavia Librayanti Vierdina Piay*3
  4. Chandra Nourmasari Ramadani*4
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Mahasiswa Fakultas Farmasi
  4. Mahasiswa Fakultas Farmasi

Abstract :

The research aim was to investigate preparation and characterisation of ovalbumin-loaded alginate microspheres produced by aerosolisation and study its in vitro release. Calcium chloride was use as crosslinking agent with crosslinking time of 30 and 120 minutes. Aerosolization technique offers a simple, fast, produce homogenous microspheres and a safe technique with no organic solvent involved. Ovalbuin-loaded-alginate microspheres were evaluated in terms of size, morphology, encapsulation efficiency, loadings of protein and yield of microspheres. In vitro release study was conducetd in simulated gastric fluid (HCl pH 1.2) and simulated intestinal fluid (PBS pH 7.4). Results showed that smaller particle size (12-23 µm) and higher encapsulation efficiency, loading and yield was produced by inceasing alginate and crosslinker concentration as well as increasing crosslinking time from 30 minutes to 120 minutes. Furthermore, by increasing alginate concentration and crosslinking time, release of ovalbumin from alginate microspheres decreased and slower in pH 1,2 during 2 hours incubationfollowed by 6 hours incubation in PBS pH 7,4. These findings indicated its potential for oral protein delivery system.  

Keyword :

alginate microspheres, ovalbumin, crosslinking time, aerosolization, release,


References :

Benoit, M.-A., Baras, B., Gillard, J,(1999) Preparation and characterization of protein-loaded poly(o-caprolactone) microparticles for oral vaccine delivery - : -

Coradin, T., Livage, J,(2003) Synthesis and Characterization of Alginate/Silica Biocomposites - : -

Croguennec, T., Renault, A., Beaufils, S., Dubois, J., Pezennec, S,(2007) Interfacial properties of heat-treated ovalbumin - : -

Draget, K.I., Smidsred, Olav., Skjak-Braek, G,(2005) Alginate from Algae. - : -





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2013-07
  1. Pelepasan Dan Penetrasi Natrium Diklofenak Sistem Niosom Span 60 Dalam Basis Gel Hpmc 4000
  2. Pengaruh Formulasi Terhadap Efekifitas Antimikroba Ekstrak Etanol 70% Daun Cassia Alata Linn Pada Candida Albicans
  3. Effect Of Hpmc K4m On Lactobacillus Acidophillus Viability In Tablet Dosage Form
  4. Optimasi Mikrosfer Ovalbumin-alginat Yang Diproduksi Dengan Teknik Aerosolisasi
  5. Pengaruh Gliserin Dan Propilenglikol Terhadap Karakteristik Fisik, Kimia Dan Spf Sediaan Krim Tipe O/w Ekstrak Biji Kakao (theobroma Cacao L.) (kadar Ekstrak Kakao 10%, 15% Dan 20%)

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