UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 2 / Published : 2011-07

Order : 5, and page :105 - 110

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Original Article :

Activity test of gynura procumbens leaves extract as antiangiogenic on chick embryo chorioallantoic membrane induced by basic fibroblast growth factor (bfgf)

Author :

  1. Hamid, I.S*1
  2. Wahyuni, R.S*2
  3. Bijanti, Retno*3
  4. Maslachah, Lilik*4
  5. Yuliati, G.A*5
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga
  5. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Angiogenesis supplies oxygen and nutrition for cancer cells in order to fulfill their needs to keep growing. So, a blockade of angiogenesis is a promising strategy to suppress tumor growth, invasion, and metastasis. Flavonoid which are concentrated in the exract of Gynura procumbens leaves are widely known has anti angiogenic effect. The chick CAM (Chorio Allantoic Membrane) methods was used for this aim. Eggs at the age of nine days were divided into 6 groups. Two groups are control: bFGF and vehicle. The next four groups are extract of Gynura procumbens leaves that variate in 4 dossage: 60, 75, 90 and 110 μg. At the age of twelve, macroscopic and microscopic analysis was done. Macroscopicly, the extract group can inhibit the new blood vessels formation. This fact is supported by microscopic analysis. Based on haematoxylin-eosin staining, angiogenic blood vessel in the extract group was less than the control bFGF group.The results showed that the extract of Gynura procumbens leaves could inhibit angiogenesis in a dose-dependent manner. Doses 60, 75, 90 and 110 μg gave angiogenesis response of 242.50 ± 69.63; 144.00 ± 15.30; 92.75 ±5.38 and 70.25 ± 13.07.

Keyword :

Antiangiogenic, CAM, Gynura procumbens,


References :

Ribatti, D., A. Gulandris, M. Bastaki, A. Vacca, M. Iurlaro, L. Roncali and M. Presta.,(1997) New Model for the Study of Angiogenesis and Antiangiogenesis in the Chick Embryo Chorioallantoic Membrane : The Gelatin Sponge / Chorioallantoic Membrane Assay United States : Vascular Research, 34:455-463.

Liu, W., Y. Yu, R. Yang, C. Wan, B. Xu and S. Cao,(2010) Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis United States : Int. J. Mol. Sci. 2010, 11, 47504763; doi:10.3390/ijms11114750.

Chen, Y., X.X. Li, N.Z. Xing, and X.G. Cao,(2008) Quercetin inhibits choroidal and retinal angiogenesis in vitro United States : Graefes Arch Clin Exp Ophthalmol.





Archive Article

Cover Media Content

Volume : 4 / No. : 2 / Pub. : 2011-07
  1. The Effect Of Tyrosine Kinases Addition Into The Egg Yolk Skim And Egg Yolk Tris Extender The Motility And Live Spermatozoa Percentages Of The Holstein Friesian Post Thawing
  2. Avian Influenza H5 Virus Excretion Post Challenge Test Of Chickens Immunized With Avian Influenza (h5n1) Virus Indonesian Strain And Commercial Heterologous Vaccine
  3. Reepithelialization And Ck 16 Expression On Superficial-thickness Wound With Topical Hyaluronate And Freeze-dried Amniotic Membrane Treatment
  4. The Effects Of Various Concentration Propanediol Cryoprotectan At Vitrification Process To Viability Of Mice Embryos Post Thawing
  5. Activity Test Of Gynura Procumbens Leaves Extract As Antiangiogenic On Chick Embryo Chorioallantoic Membrane Induced By Basic Fibroblast Growth Factor (bfgf)
  6. Efect Of Centrifugation Of Goat Semen To Free Radical Concentrate On Washing Medium
  7. Isolation Identification And Sensitivity Test Of Bacillus Subtilis From Shrimp Culture Ponds To Antimicrobial Agents
  8. The Differences In Several Parameters Of The Femur Bone Related To The Biomechanics Of The Bucks And Doe
  9. The Influence Of Temperature On Meat Decay One Of Beef Sold The Traditional Market
  10. Antibody Response And Protectivity In Chickens After Vaccination With Nd Lv12 Active Vaccine
  11. Concentration Of Pregnancy Associated Glycoprotein (pag) Of Milk Of Dairy Cows At 21 And 28 Days Post Artificial Insemination
  12. The Increase Of Activated Goblet Cells In The Duodenum Of Broiler Exposed By Heat Stress
  13. (z)-9- Heptacosene Sex Pheromone Level Production On Cuticular Hydrocarbon Field Of Strain Musca Domestica
  14. Antibacterial Potency Of Honey And Noni Juice (morinda Citrifolia L.) To The Number Of Staphylococcus Aureus On Chicken Carcasses
  15. Mengukur Kadar Protein Fertility Associated Antigen (faa) Pada Semen Sapi Menggunakan Qubit Fluorometer

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