UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 7 / No. 2 / Published : 2014-07

Order : 2, and page :106 - 113

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Original Article :

Antibacterial activity of the supernatant of soil isolate bacillus subtilis against aeromonas hydrophila and staphylococcus aureus (in vitro)

Author :

  1. Erni Rosilawati Sabar Iman*1
  2. Mirni Lamid*2
  3. Madya Adi Waskita*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Mahasiswa Fakultas Kedokteran Hewan

Abstract :

The aim of this research was to know the antibacterial activity (Minimum Inhibitory Concentration and Minimum Bactericidal Concentration) of supernatant of soil isolate Bacillus subtilis against Aeromonas hydrophila and Staphylococcus aureus and compare the antibacterial activity both of them. Antibacterial compound from B. subtilis are subtilin, subtilosin A, TasA, sublancin, circulin, polymixin and colistin which effective against Gram positive and Gram negative bacteria. Microdilution method was used to evaluate the antibacterial activity from supernatant of Bacillus subtilis by determining MIC and MBC. Concentration range was from 100% - 10%. The result showed the range of MIC and MBC values against Aeromonas hydrophila were 60% - 70% and 70% - 80%. Meanwhile, the range of MIC and MBC values against Staphylococcus aureus were 80% - 90% and 90% - 100%. Statistical analyzed showed that there were highly significant difference of antibacterial activity of supernatant of soil isolate Bacillus subtilis against Aeromonas hydrophila as Gram negative and Staphylococcus aureus as Gram positive bacteria.

Keyword :

Bacillus subtilis, Antibacterial activity, Aeromonas hydrophila, Staphylococcus aureus,


References :

Aslim, B., S. Neode and B. Yavuz,(2002) Determination of some properties of Bacillus isolated from soil Istanbul : Turkish Journal of Biology.41-48.

Atyah, M.A.S., M.Z. Saad and A.S. Zahrah,(2010) First report of methicillin-resistant Staphylococcus aureus from cage-cultured tilapia (Oreochromis niloticus). . : Veterinary Microbiology 144:502-504

Awais, M., A.A. Shah, A. Hammed and F. Hasan,(2007) Isolation, identification and optimization of Bacitracin produced Bacillus sp . : Pak. J. Bot., 39(4): 1303-1312





Archive Article

Cover Media Content

Volume : 7 / No. : 2 / Pub. : 2014-07
  1. The Influence Of Carbofuran Exposure Toward White Pulp Diameter Of Spleen Of Mice (mus Musculus)
  2. Antibacterial Activity Of The Supernatant Of Soil Isolate Bacillus Subtilis Against Aeromonas Hydrophila And Staphylococcus Aureus (in Vitro)
  3. The Potential Of Giving Synbiotic In Different Ages Of Female Broilers On Histological Of Ileum
  4. Evaluation Toluidine Blue Staining To Identify Connective Tissue Mast Cells (ctms) In Paraffin Block Thin Skin Of Dog
  5. Effect Using A Combination Of Mpa (medroxy Progesterone Acetate) And Prostaglandin (pgf2α) Injection On The Percentage Of Estrous And Pregnant On Sheeps
  6. Spesifisity Test With Dot Blotting Of Epidermal Growth Factor (egf) Isolated From Cumulus Oocyte Complex After In Vitro Maturation
  7. Analysis Of Immunogenicity On Inactivated Dengue Virus (denv-1, Denv-2, Denv-3, Denv-4) In Mices (mus Musculus) As A Candidate Dengue Coctail Vaccine
  8. Detection Antibody Of Brucella On Cattle Slaughtered In Krian Slaughter House Sidoarjo Regency By Rose Bengal Test (rbt)
  9. The Effect Of Didecyldimethylammonium Chloride Disinfectants On Liver Histophatolologycal Of Duck Hybrid (anas Platyrhynchos Domesticus)
  10. The Effect Of Cosmos Caudatus Leaf Ethanol Extract On Paracetamol Induced In Histopathologic Liver Of (mus Musculus) Balb / C Male
  11. Antibacterial Test Of Rumbia Root (metroxylon Sagu Rottb.) Decoction Against Bacteria Salmonella Pullorum
  12. Effect Of Sambiloto Leaf Extract (andrographis Paniculata Ness) To Histopathological Pancreatic Langerhans Islet Cells On Rats (rattus Norvegicus) With Cystic Ovary Model
  13. Effect Of Mangosteen (garcinia Mangostana L.) Pericarp Extract On Tlr5 And Cd14 Expression In Immunized Mice Against Newcastle Disease Vaccine
  14. Cloning Gene Fragments Non-structural 1 (ns1) Of Dengue Virus Subtype 1 (denv-1) As A Material Candidate Of Vaccine Chimera
  15. Effect Of Vitamin E (α–tocopherol) On The Number Of Leydig Cell In Mice Treated With 2,3,7,8–tetrachlorodibenzo-p-dioxin (tcdd)

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