Veterinaria Medika
ISSN 1979-1305
Vol. 4 / No. 2 / Published : 2011-07
Order : 2, and page :91 - 94
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Original Article :
Avian influenza h5 virus excretion post challenge test of chickens immunized with avian influenza (h5n1) virus indonesian strain and commercial heterologous vaccine
Author :
- Rahardjo, A.P.*1
- Anindyaputri, Ghea*2
- Estoepangestie, A.T.S.*3
- Mustofa, Imam*4
- Fakultas Kedokteran Hewan Universitas Airlangga
- PPDH Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
Abstract :
The aim of the study was to detect Avian Influenza H5 viral shedding in chickens post immunized using Indonesian virus isolates and commercial vaccines, after challenge using Indonesian Avian Influenza H5N1 isolates. Six groups of chickens were vaccinated, five groups using several Indonesian virus isolates Avian Influenza H5N1, while the remaining group using commercial vaccine. Four weeks after the last booster vaccination, all groups of chickens were challenged with A/Ck/Indonesia/BL/2003 (H5N1) virus isolates. At day 1 to 8 post challenge, cloacal swabs were taken followed by isolation in 8 to 10 days old embryonated eggs and confirm using HA and HI test. The result showed that Avian Influenza fecal viral shedding in vaccinated group was low to completely negative, while in control group could be detected from second up to fourth days post challenged until the death of chickens.
Keyword :
Viral shedding, Avian Influenza Indonesian virus isolates, Cloacal swabs,
References :
Lebdah, M.A., and Shahin, A. M.,(2010) Evaluation of Avian Influenza Vaccines Used in Broiler Flocks in Egypt. Avian and Rabbit Medicine Egypt : Departement, Faculty of Vet. Med.: Zagazig University.6 (12).
L.P. Michael, R.B. Joan, G. Maricarmen, and L.S. David.,(2000) Vaccines Protect Chicken Against H5 Highly Pathogenic Avian Influenza in The Face of Genetic Change in Field Viruses Over Multiple Years United States : Veterinary Microbiology. 74: 165-172
Radji, M,(2006) Avian Influenza A (H5N1): Patogenesis, Pencegahan, dan Penyebaran Pada Manusia Jakarta : Majalah Ilmu Kefarmasian, Vol 3, No 2
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