UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 3 / Published : 2015-11

Order : 6, and page :279 - 284

Related with : Scholar   Yahoo!   Bing

Original Article :

Comparison of antibody titre of anti brucella abortus using cft and ielisa to cattle with positive rbt

Author :

  1. Eni Rohyati*1
  2. Suwarno*2
  3. AT.Soelih Estoepangestie*3
  1. Politeknik Pertanian Negeri Kupang
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The purpose of this research was aimed to compare the performance of indirect ELISA (iELISA) test using LPS antigen of B.abortus S19 and Complement Fixation Test (CFT) using whole cell antigen of B.abortus S99 to detect antibody B.abortus in serum of cattle in Belu Regency. A total of 251 serum samples were collected randomly from cattle ranches in Belu Regency which includes of 42 males and 209 females. The sera have been analyzed using Rose Bengal Test (RBT). All 17 RBT positive results were further tested by iELISA test using LPS antigen of B.abortus S19 and CFT using whole cell antigen of B.abortus S99. The results showed that the direct and indirect regression coefficients (β coefficients) from RBT to iELISA test (0.839 and 1.128) were stronger than the direct and indirect regression coefficients from RBT to CFT (0.580 and 0.998), and that there was an ideal aggrement (Kappa = 1) of iELISA test using LPS antigen of B.abortus S19 with CFT using whole cell antigen of B.abortus S99.

Keyword :

Brucella, LPS, iELISA, CFT, RBT,


References :

Hewitt, W G and D.J. Payne,(2015) Estimation of IgG and IgM Brucella Antibodies in Infected and Non-Infected Persons by a Radioimmune Capture 37: 692-696 : J Clin Pathol

Özdemir, M., F. Bahadır, G.K. Muhammed, D. Metin, T.D. Hatice, Y. Şerife, K. Recep, B. Bülent,(2011) A Comparison of Immuncapture Agglutination and ELISA Methods in Sero-logical Diagnosis of Brucellosis (5):428-432 : International Journal of Medical Sciences

Ragan, V., G. Vroegindewey, S. Babcock,(2013) International Standars for Brucellosis Prevention and Management 32 (1): 189-198 : Rev.sci.tech.Off.int. Epiz





Archive Article

Cover Media Content

Volume : 8 / No. : 3 / Pub. : 2015-11
  1. Genotyping Viral Nervous Necrosis (vnn) In Groupers In East Java And West Nusa Tenggara
  2. Lactoferrin Effect On Biofilm Formation Of Klebsiella Pneumoniae Isolated From Mastitis Cases Of Dairy Cows
  3. Effect Of Insulin Transferrin Selenium (its) Supplementation In Maturation Medium Tcm-199 To Bovine Oocyte Diameter
  4. Supplementation Of Insulin Transferrin Selenium On In Vitro Maturation Cumulus Oocyte Complex (coc) Against To Mitogen Activated Protein Kinase (mapk) Expression And Expanded Cumulus Mass
  5. Adaptation Capability And Antigen Stability Of Newcastle Disease Virus Local Isolate On Hela Cell
  6. Comparison Of Antibody Titre Of Anti Brucella Abortus Using Cft And Ielisa To Cattle With Positive Rbt
  7. Potency Of Apple Vinegar As Alternatif Therapy Against Wound Healing Time Of Staphylococcus Aureus Infection On Mice
  8. Suplementation Of Specific Protein Membrane Spermatozoa In Freezing Medium To Improvement Efforts A Succes Cattle Frozen Semen Quality Post Thawing
  9. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat After Infected By Salmonella Typhimurium
  10. The Potential Of Outer Membrane Protein Brucella Abortus Local Isolate As Brucellosis Vaccine Development In Indonesia
  11. The Using Of Whole Serum Pmsg (pregnat Mare Serum Gonadotropin) With Sepadex Extracted For Increasing Estrus Induction Of Ettawa Goat
  12. Toxicity Test Of Singawalang (petiveria Alliaceae) Leaves Extract To The Hypertrophy Of Male White Mice (mus Musculus) Cardiac Muscle
  13. Effect Of Benzo(a)pyrene Injection To The Initiation Of Mammae Cancer At Mice

Information index.php/JIPK/VETMED