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Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 9 / No. 2 / Published : 2016-07

Order : 5, and page :125 - 130

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Original Article :

Detection gene coding capsule type a of pasteurella multocida from cases of fowl cholera by polymerase chain reaction

Author :

  1. Faulanni Adianty Fajrin*1
  2. Didik Handijatno*2
  3. Thomas V Widiyatno*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this study was to identify the presence of gene capsule type A of Pasteurella multocida from cases of fowl cholera by Polymerase Chain Reaction. The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. However, such tests are laborious, and agglutination of homologous antiserum may fail. The samples taken from visceral organs such as liver and heart blood of birds that succumb to the acute bacteraemic form of the disease. Thirty sample organs were used in this study. First, The samples for the isolation of P. multocida were macerated and plated on 5% sheep blood agar and MacConkey agar, incubated at 37°C for 48 hours in aerobiosis, followed by the morphological and biochemical characterization. PCR based on the Pasteurella multocida gene Capsular was done to confirm the isolate as previously described. The result showed that 2 of 30 samples were positive Pasteurella multocida. By PCR assay there was no positive samples confirmed as gene coding Capsular.

Keyword :

Pasteurella multocida, fowl cholera, capsule type A, Polymerase Chain Reaction.,


References :

Boyce, J.D., Harper, M., Wilkiie, I.W., and Adler, B.,(2010) Pasteurella In: Pathogenesis of Bacterial Infection In animal 4th Ed. Gyles C.I., Prescott,J.F., Songer,G., and Thoen C.O.(Eds) 325-346 : Blackwell Publishing, Ames, Iowa, USA.

Chung, J.Y., Wilkie, I., Boyce, J.D., Townsend, K.M., Frost, A.J, Adler, B., and Ghoddusi, M.,(2001) Role of Capsule in the Pathogenesis of Fowl Cholera Caused by Pasteurella multocida Serogroup A 2487-2492 : Infection and Immunity

Fatchiyah., Arumingtyas, E.L., Widyarti, S., Rahayu, S.,(2009) Dasar-dasar Analisa Biologi Molekuler 62 : Lembaga Penerbitan Fakultas Pertanian Universitas Brawijaya. Malang





Archive Article

Cover Media Content

Volume : 9 / No. : 2 / Pub. : 2016-07
  1. Therapy Effect Of (pandanus Conoideus Inn) On Female Mice (mus Musculus) Infertility Model Toward The Folicle Diameter Size Of Pre Antral And Antral Histopathological Features
  2. The Effects Of Insulin Transferin Selenium Supplementation On Mice (mus Musculus) Embryos Vitrification Media At Morula Stage Towards The Viability Of Blastomere Cells Using Fluorescence Techniques After Warming
  3. Staphylococcus Aureus Contamination Rate On Beef Slaughter Results In Pegirian Surabaya Slaughterhouse
  4. Response Of Antibodies Formed As Result Of Brucella Suis Infection In Mice (mus Musculus) With Complement Fixation Test (cft)
  5. Therapeutic Effect Of Spirulinaplatensis Extract On Histopathological Appearance Of Ethanol Induced Liver Injury In Rat (rattus Norvegicus)
  6. Detection Gene Coding Capsule Type A Of Pasteurella Multocida From Cases Of Fowl Cholera By Polymerase Chain Reaction
  7. The Resistance Of Escherichia Coli From Pig Suspect Colibacillosis In A Pig Farm In Loa Duri Ulu Village To Antibiotics
  8. The Prevalence Of Bacteria Escherichia Coli On Meat Of Horse Slaughtered At Tumpang Abattoir In Malang District East Java
  9. Utilization Of Coconut Shell Liquid Smoke As Preservation In Chicken Intestine On The Value Of Total Plate Count
  10. Effect Of Red Fruit (pandanus Conoideus Lam.) Oil To Litter Size And Congenital Defecton Lead Intoxicated Pregnant Mice (mus Musculus)
  11. Sensitivity Test Of Some Antibiotic Types On Bacteria Of Endometritis In Pig Farm, Sukapura, Probolinggo
  12. Potential Test Of Cucumber Tree(averrhoa Bilimbi) Leaves Suspension As Alternative Mackerel (scomberomorus Sp) Preservatives
  13. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat Before Infected By Salmonella Typhimurium
  14. Influence Of Red Betel Leaf’s Extract (piper Crocatum) To The Histopatology Image On Rat’s Skin Which Infected By Staphylococcus Aureus
  15. Characteristic Morphology And Biochemical Of Cellulolytic Isolate Cytophaga Sp
  16. Red Cell Count (erythrocyte), Hematocrit, And Hemoglobin Of Catfish (clarias Gariepinus) After Infected By Aeromonas Hydrophila
  17. The Effect Of Extract Meniran (phyllanthus Niruri Linn.)to Ileum Histopathological Changes In Ratsinduced By Alcohol

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