UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 2 / No. 1 / Published : 2009-02

Order : 10, and page :49 - 54

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Original Article :

Detection of progesterone of pregnant dairy cattle using enzyme immunoassay (eia) method

Author :

  1. Abdul Samik*1
  1. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The sustainability of livestock sources is related to birth rate increase and the shortening of calving interval. One of the efforts to shorten the calving interval in dairy cattle is by conducting early diagnosis of pregnancy after mating. The development of early pregnancy diagnosis method in livestock can be undertaken by observing progesterone hormone in the blood of pregnant livestock. This study was conducted by following these stages: 1) Oestrus synconization and artificial insemination of dairy cattle, 2) Dairy cattle pregnancy diagnosis by measuring blood serum progesterone level, and 3) Pregnancy diagnosis with abdominal palpation.Progesterone titer of pregnant dairy cattle obtainen in this reseach was ranging between 1.18 –5.96ng/ml. The result of blood serum progesterone level with abdominal palpation revealed sensitivity,specificity, accuracy, positive predictive, and negative predictive values of 94,29%, 80%, 90 %, 91,67% and 85,71%.Based on the results, it can be concluded that progesterone hormone can be employed for dairycattle pregnancy diagnosis with Enzyme Immunoassay Method.

Keyword :

oestrus syncronization, artificial insemination, Progesterone, rectal palpation, .,


References :

Hafez, E.S.E. ,(2000) Reproduction in Farm Animals Philadelphia : Lippincutt Williams and Wilkins

Austin C.R and R.V. Short,(1984) Mechanisms of Hormone Action London : Cambridge University Press

Broaddus B. And A de Vries,(2005) A Comparison of Methods for Early Pregnancy Diagnosis Florida : Proceeding 2 nd Florida Dairy Road Show





Archive Article

Cover Media Content

Volume : 2 / No. : 1 / Pub. : 2009-02
  1. The Effect Of Water Extract From Phyllanthus Niruri L. Is Immunostimulator In Newcastle Disease Vaccinated Chicken
  2. Proliferation Activity Of Gland Mammae After Leaves Gynura Procumbens Extract Which Dmba (dimethylbenz(a)antrasen) Initiation On Sprague Dawley Rat
  3. Growth Differentiation Factor-9 (gdf-9) Characterization On Bovine Oocyte Collected From Preanthral Follicle Using Dotblotting Method
  4. Optical Density Value Of Antibody Against Pregnancy-associated Glycoproteins (pags) From The Pregnant Dairy Cow Blood Serum In Male Local Rabbit (oryctolagus Cuniculus)
  5. Implementation Studies Of Artificial Insemination Program Of Beef Cattle In West Java
  6. Glycoprotein Of Human Membrane Spermatozoa
  7. Specifitacy Test Anti-prostaglandin F2a Indirect Elisa Method
  8. The Sensitivity Test Of Some Antimicrobial Agents To Staphylococcus Aureus Of Cause Mastitis At Dairy Cattle At Around Of Suka Makmur Dairy Cooperative Grati Pasuruan
  9. Correlation Between Subclinical Mastitis Stages Use California Mastitis Test With Total Bacteria In Milk
  10. Detection Of Progesterone Of Pregnant Dairy Cattle Using Enzyme Immunoassay (eia) Method
  11. Identification Of Encoding Gene For Fibrinogen Binding Protein On Staphylococcus Aureus In Mastitic Milk
  12. The Polymorphism Of Coagulase Gene As Searching Strain Variability Of Staphylococcus Aureus From Bovine Mastitis Cases
  13. Concentration Of Total Protein From Goat Seminal Plasma By Biuret Method
  14. The Consumption Of Crude Protein Of Haylage Complete Feed On Ongole Crossbreed Steers
  15. Crude Protein Contents And Dry Matter In Ipome Sp Fermentation As Alternative For Feed Stuff
  16. The Effect Of Forced Moulting In The Avian As Causes Of Zoonocis Case Salmonella Enteridis (se)

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